MHC class II Antibody (15G4)

MHC class II Antibody (15G4): sc-53946

MHC class II Antibody (15G4) is rated 4.0 out of 5 by 2.
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Datasheets

    • MHC class II Antibody (15G4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against LPS B cell blasts of H-2M knockout mouse origin
    • recommended for detection of MHC class II molecule I-Ab bound to invariant chain (Ii) degradation intermediates p12 and class II-associated invariant chain peptides (CLIP) of mouse origin; not recommended for detection of MHC class II molecule I-Ab bound to human invariant chain degradation intermediates of mouse origin by IP, IF and FCM; not recommended for detection of MHC class II molecule I-Ab bound to human invariant chain degradation intermediates
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Can MHC class II (15G4): sc-53946 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, MHC class II (15G4): sc-53946 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Works ok on western blot Works on ok western blot. I cut the membrane so I do not know how non specific they are, so I am giving 4 stars which is still a very high rating.
Date published: 2017-08-01
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