MHC class II Antibody (15G4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
raised against LPS B cell blasts of H-2M knockout mouse origin
recommended for detection of MHC class II molecule I-Ab bound to invariant chain (Ii) degradation intermediates p12 and class II-associated invariant chain peptides (CLIP) of mouse origin; not recommended for detection of MHC class II molecule I-Ab bound to human invariant chain degradation intermediates of mouse origin by IP, IF and FCM; not recommended for detection of MHC class II molecule I-Ab bound to human invariant chain degradation intermediates
available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Major histocompatibility complex (MHC) molecules, also designated human leukocyte antigen (HLA) molecules, are cell-surface receptors that bind foreign peptides and present them to T lymphocytes. MHC class I molecules consist of two polypeptide chains, an α or heavy chain and β-2-Microglobulin, a non-covalently associated protein. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically eight to ten residues in length and are stabilized in a peptide binding groove. MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an α and β chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumu-lating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Can MHC class II (15G4): sc-53946 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?
Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, MHC class II (15G4): sc-53946 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Rated 4 out of
Works ok on western blotWorks on ok western blot. I cut the membrane so I do not know how non specific they are, so I am giving 4 stars which is still a very high rating.
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