MHC class I Antibody (W6/32) recommended for detection of HLA-A, HLA-B and HLA-C of human origin by WB, IP, IF and FCM

MHC class I Antibody (W6/32): sc-32235

MHC class I Antibody (W6/32) is rated 4.3 out of 5 by 14.
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Datasheets

    • Anti-MHC class I Antibody (W6/32) is a mouse monoclonal IgG2a MHC class I antibody provided at 200 µg/ml
    • MHC class I Antibody (W6/32) is recommended for detection of HLA-A, HLA-B and HLA-C of human origin by WB, IP, IF and FCM
    • recognizing the W6/32 antigenic determinant common to HLA-A, B and C antigens of human origin
    • Anti-MHC class I Antibody (W6/32) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either PerCP or PerCP-Cy5.5 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MHC class I (W6/32): sc-32235.
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Are the agarose conjugated antibodies covalently bound ? Do they resist to acidic elution for instance and keep attached to the agarose? Thx

Asked by: YannE
Thank you for your question. Yes, they are covalently bound via the primary amine. I do not have information specifically regarding acidic elution.
Answered by: Tech Service
Date published: 2020-01-17

Is it your W6/32 Clone from ATCC? And which type of WB could be applied with your "Anticorps MHC class I (W6/32): sc-32235", reducing/denaturing condition or non-reducing condition? Thanks

Asked by: Yang1109
Thank you for your question. We recommend reducing/denaturing conditions with this antibody for Western blotting.
Answered by: Tech Service
Date published: 2019-04-16

can we use this antibody against mouse MHC I because it is recommended against Human MHC I prtotein

Asked by: junaid
Thank you for your question. This antibody, MHC class I (W6/32): sc-32235 , has not been tested for and is not recommended for detection of mouse MHC I.
Answered by: Technical Support
Date published: 2018-01-31

I want an antigen which can not react with β2 microglobulin existing in the β chian. Does it react with all regions of MHC class I, or just react with α region?

Asked by: Sarah Wang
Thank you for your question. This antibody recognizes the W6/32 antigenic determinant common to HLA-A, B and C antigens of human origin. The exact epitope for this antibody has not been epitope mapped, so we do not have a more detailed answer. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2018-01-15

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2016-12-23
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Rated 5 out of 5 by from FACS on HFF This antibody works very well in Flow cytometry on HFF cells even at dilution 1:50
Date published: 2018-11-01
Rated 4 out of 5 by from Good antibody for western blot I cheked MHC class 1 expression in MCF7, MB453 and A375 cell lysates by western blot. I used it 1:500 dilution, O/N at 4 C. I got a clear band at 46kDa.
Date published: 2018-10-01
Rated 1 out of 5 by from Not good with IHC We didn't receive a signal with IHC. We tried no demasking and demasking with pH 6 and 9. We stained human tonsils and brain (5 µm FFPE).
Date published: 2018-04-12
Rated 5 out of 5 by from Good antibody for flow cytometry I got a free sample of this antibody for a flow cytometry test and it worked really well. I used human fibroblast cell lines (Hs27) and Hela cells. The antibody concentration was 1:500.
Date published: 2017-12-28
Rated 5 out of 5 by from good antibody (1/200)WB experiment:I got a very clear band. Recommended for use!
Date published: 2017-09-07
Rated 4 out of 5 by from Works on embryonic mouse brain tissue, too. I got this as a free sample from Santa Cruz and tested on both embryonic rat and mouse brain tissue, it works great on mouse brain tissue but not the rat. I used 1:500 over night at 4 C.
Date published: 2017-01-23
Rated 5 out of 5 by from good signal Strong band in WB in human various breast cancer cell lines, good signal.
Date published: 2017-01-05
Rated 5 out of 5 by from single band! it is very specific. i used it for C2C12 and at 46kDa there's a strong band!
Date published: 2017-01-05
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