MHC class I Antibody (VPM 19) recommended for detection of MHC class I in lymphocytes isolated from either peripheral blood or efferent lymph of ovine and bovine origin by WB, IP, IF and FCM

MHC class I Antibody (VPM 19): sc-59205

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Datasheets

    • MHC class I Antibody (VPM 19) is a mouse monoclonal IgG1 (lambda light chain) provided at 200 µg/ml
    • raised against thymocytes of ovine origin
    • recommended for detection of MHC class I in lymphocytes isolated from either peripheral blood or efferent lymph of ovine and bovine origin by WB, IP, IF and FCM; not recommended for detection of lymphocytes in red blood cells, thymus and brain
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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How can MHC class I (VPM 19): sc-59205 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Trav11
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-59205 PE) or fluoroscein (sc-59205 FITC).
Answered by: Technical Support
Date published: 2017-02-27
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