MFF Antibody (F-6): sc-515648

MFF Antibody (F-6) is a mouse monoclonal IgG1 antibody that detects MFF (mitochondrial fission factor) in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MFF is a crucial 342 amino acid single-pass type IV membrane protein located in the outer membrane of mitochondria, playing a vital role in mitochondrial and peroxisomal fission, which is essential for maintaining cellular energy homeostasis and regulating apoptosis. The proper functioning of MFF facilitates the dynamic remodeling of mitochondria, allowing cells to adapt to metabolic demands and stress conditions. MFF is expressed abundantly in various tissues, including the stomach, heart, muscle, liver, brain, and kidney, and exists as five alternatively spliced isoforms encoded by a gene located on human chromosome 2q36.3. This chromosome is significant as it is the second largest in the human genome, containing over 1,400 genes and being associated with several genetic diseases. MFF (F-6) antibody provides a reliable tool for researchers studying mitochondrial dynamics and related pathologies, enabling insights into the molecular mechanisms underlying various diseases linked to mitochondrial dysfunction.

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MFF Antibody (F-6) References:

1. Targeting and function of the mitochondrial fission factor GDAP1 are dependent on its tail-anchor.  |  Wagner, KM., et al. 2009. PLoS One. 4: e5160. PMID: 19340293
2. Mitochondrial fission factor Drp1 is essential for embryonic development and synapse formation in mice.  |  Ishihara, N., et al. 2009. Nat Cell Biol. 11: 958-66. PMID: 19578372
3. The mitochondrial fission factor dynamin-related protein 1 modulates T-cell receptor signalling at the immune synapse.  |  Baixauli, F., et al. 2011. EMBO J. 30: 1238-50. PMID: 21326213
4. Depletion of mitochondrial fission factor DRP1 causes increased apoptosis in human colon cancer cells.  |  Inoue-Yamauchi, A. and Oda, H. 2012. Biochem Biophys Res Commun. 421: 81-5. PMID: 22487795
5. miR-761 regulates the mitochondrial network by targeting mitochondrial fission factor.  |  Long, B., et al. 2013. Free Radic Biol Med. 65: 371-379. PMID: 23867156
6. miR-27 regulates mitochondrial networks by directly targeting the mitochondrial fission factor.  |  Tak, H., et al. 2014. Exp Mol Med. 46: e123. PMID: 25431021
7. Distinct Splice Variants of Dynamin-related Protein 1 Differentially Utilize Mitochondrial Fission Factor as an Effector of Cooperative GTPase Activity.  |  Macdonald, PJ., et al. 2016. J Biol Chem. 291: 493-507. PMID: 26578513
8. Removal of the Mitochondrial Fission Factor Mff Exacerbates Neuronal Loss and Neurological Phenotypes in a Huntington's Disease Mouse Model.  |  Cha, MY., et al. 2018. PLoS Curr. 10: PMID: 30279997
9. Clinical and Biochemical Features in a Patient With Mitochondrial Fission Factor Gene Alteration.  |  Nasca, A., et al. 2018. Front Genet. 9: 625. PMID: 30581454
10. Parkin-mediated ubiquitination contributes to the constitutive turnover of mitochondrial fission factor (Mff).  |  Lee, L., et al. 2019. PLoS One. 14: e0213116. PMID: 31112535

The preferred MFF antibody is MFF Antibody (B-2): sc-398617.