MFF Antibody (B-2): sc-398617

MFF Antibody (B-2) is a mouse monoclonal IgG3 kappa light chain antibody that detects MFF of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-MFF antibody (B-2) is available as the non-conjugated form. MFF, or mitochondrial fission factor, plays a crucial role in regulating mitochondrial and peroxisomal fission, processes essential for maintaining cellular energy homeostasis and overall cellular health. MFF is predominantly located in the outer membrane of mitochondria, where MFF facilitates the recruitment of dynamin-related protein 1 (Drp1), a key player in mitochondrial division. This interaction is vital for proper distribution of mitochondria during cell division and for response to cellular stress, highlighting MFF′s importance in both normal cellular function and in the pathogenesis of various diseases, including neurodegenerative disorders. MFF exists as five alternatively spliced isoforms, which adds complexity to functional roles and regulatory mechanisms. The gene encoding MFF is located on human chromosome 2q36.3, a region associated with several genetic diseases, underscoring MFF′s potential implications in health and disease.

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MFF Antibody (B-2) References:

1. Alstrom syndrome: confirmation of linkage to chromosome 2p12-13 and phenotypic heterogeneity in three affected sibs.  |  Zumsteg, U., et al. 2000. J Med Genet. 37: E8. PMID: 10882760
2. An ATP-binding cassette gene (ABCG5) from the ABCG (White) gene subfamily maps to human chromosome 2p21 in the region of the Sitosterolemia locus.  |  Shulenin, S., et al. 2001. Cytogenet Cell Genet. 92: 204-8. PMID: 11435688
3. Mutation of ALMS1, a large gene with a tandem repeat encoding 47 amino acids, causes Alström syndrome.  |  Hearn, T., et al. 2002. Nat Genet. 31: 79-83. PMID: 11941370
4. Mutations in ABCA12 underlie the severe congenital skin disease harlequin ichthyosis.  |  Kelsell, DP., et al. 2005. Am J Hum Genet. 76: 794-803. PMID: 15756637
5. The novel tail-anchored membrane protein Mff controls mitochondrial and peroxisomal fission in mammalian cells.  |  Gandre-Babbe, S. and van der Bliek, AM. 2008. Mol Biol Cell. 19: 2402-12. PMID: 18353969
6. Differential expression of a set of genes in follicular and classic variants of papillary thyroid carcinoma.  |  Igci, YZ., et al. 2011. Endocr Pathol. 22: 86-96. PMID: 21509594
7. Mitochondrial Fission Factor (MFF) Inhibits Mitochondrial Metabolism and Reduces Breast Cancer Stem Cell (CSC) Activity.  |  Sánchez-Alvarez, R., et al. 2020. Front Oncol. 10: 1776. PMID: 33194575
8. Mitochondrial fission factor (Mff) is required for organization of the mitochondrial sheath in spermatids.  |  Varuzhanyan, G., et al. 2021. Biochim Biophys Acta Gen Subj. 1865: 129845. PMID: 33476744
9. Mitochondrial fission factor promotes cisplatin resistancein hepatocellular carcinoma.  |  Li, X., et al. 2022. Acta Biochim Biophys Sin (Shanghai). 54: 301-310. PMID: 35538029
10. The role of mitochondrial fission factor in podocyte injury in diabetic nephropathy.  |  Xiao, M., et al. 2022. Biochem Biophys Res Commun. 624: 40-46. PMID: 35932578
11. Mitochondrial fission factor (MFF) frameshift variant in Bullmastiffs with mitochondrial fission encephalopathy.  |  Christen, M., et al. 2022. Anim Genet. 53: 814-820. PMID: 36085405
12. Mapping a gene involved in regulating dietary cholesterol absorption. The sitosterolemia locus is found at chromosome 2p21.  |  Patel, SB., et al. 1998. J Clin Invest. 102: 1041-4. PMID: 9727073

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MFF Antibody (B-2) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-MFF Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MFF Antibody (B-2) Specifications

Alternate Product Name: MFF

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 91-117

Epitope Target: MFF

Epitope Species: human

Product Reactivity/Detection: MFF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for MFF Antibody (B-2): P: Blocking Peptide