megsin Antibody (H-10): sc-515694
- megsin Antibody (H-10) is a mouse monoclonal IgG1 κ megsin antibody provided at 200 µg/ml
- raised against amino acids 32-165 mapping near the N-terminus of megsin of human origin
- megsin Antibody (H-10) is recommended for detection of megsin of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-megsin Antibody (H-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for megsin Antibody (H-10) for WB applications. These reagents are now offered in bundles with megsin Antibody (H-10) (see ordering information below).
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Megsin Antibody (H-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects megsin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-megsin antibody (H-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Megsin, also known as SerpinB7 and TP55, is a 380 amino acid cytoplasmic protein predominantly expressed in mesangial cells, which are crucial for maintaining glomerular structure and function. As a member of the serpin family, megsin acts as a serine protease inhibitor, with the ability to potentially inactivate matrix metalloproteinases MMP-2 and MMP-9, as well as plasmin, all of which are key players in the degradation of the extracellular matrix. The regulation of these proteases is vital, as their activity can significantly impact tissue remodeling and repair processes. Notably, overexpression of megsin leads to an increase in the number of mesangial cells, resulting in progressive mesangial matrix expansion and immune complex deposition, which suggests that megsin plays a significant role in the pathology of various nephropathies by influencing mesangial cell function and renal structure.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
megsin Antibody (H-10) References:
- Overexpression of the serpin megsin induces progressive mesangial cell proliferation and expansion. | Miyata, T., et al. 2002. J Clin Invest. 109: 585-93. PMID: 11877466
- Association of MEGSIN 2093C-2180T haplotype at the 3' untranslated region with disease severity and progression of IgA nephropathy. | Xia, Y., et al. 2006. Nephrol Dial Transplant. 21: 1570-4. PMID: 16431886
- A severe diabetic nephropathy model with early development of nodule-like lesions induced by megsin overexpression in RAGE/iNOS transgenic mice. | Inagi, R., et al. 2006. Diabetes. 55: 356-66. PMID: 16443768
- A family-based association study of megsin A23167G polymorphism with susceptibility and progression of IgA nephropathy in a Chinese population. | Xia, YF., et al. 2006. Clin Nephrol. 65: 153-9. PMID: 16550745
- The influence of two megsin polymorphisms on the progression of IgA nephropathy. | Maixnerová, D., et al. 2008. Folia Biol (Praha). 54: 40-5. PMID: 18498720
- The role of megsin, a serine protease inhibitor, in diabetic mesangial matrix accumulation. | Ohtomo, S., et al. 2008. Kidney Int. 74: 768-74. PMID: 18580857
- Megsin gene: its genomic analysis, pathobiological functions, and therapeutic perspectives. | Miyata, T., et al. 2007. Curr Genomics. 8: 203-8. PMID: 18645605
- Megsin 2093T-2180C haplotype at the 3' untranslated region is associated with poor renal survival in Korean IgA nephropathy patients. | Lim, CS., et al. 2008. Clin Nephrol. 70: 101-9. PMID: 18793525
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
megsin Antibody (H-10) | sc-515694 | 200 µg/ml | $322.00 | |||
megsin Antibody (H-10): m-IgG Fc BP-HRP Bundle | sc-531470 | 200 µg Ab; 10 µg BP | $361.00 | |||
megsin Antibody (H-10): m-IgGκ BP-HRP Bundle | sc-525166 | 200 µg Ab, 40 µg BP | $361.00 | |||
megsin Antibody (H-10): m-IgG1 BP-HRP Bundle | sc-544574 | 200 µg Ab; 20 µg BP | $361.00 | |||
megsin Antibody (H-10) AC | sc-515694 AC | 500 µg/ml, 25% agarose | $424.00 | |||
megsin Antibody (H-10) HRP | sc-515694 HRP | 200 µg/ml | $322.00 | |||
megsin Antibody (H-10) FITC | sc-515694 FITC | 200 µg/ml | $336.00 | |||
megsin Antibody (H-10) PE | sc-515694 PE | 200 µg/ml | $349.00 | |||
megsin Antibody (H-10) Alexa Fluor® 488 | sc-515694 AF488 | 200 µg/ml | $364.00 | |||
megsin Antibody (H-10) Alexa Fluor® 546 | sc-515694 AF546 | 200 µg/ml | $364.00 | |||
megsin Antibody (H-10) Alexa Fluor® 594 | sc-515694 AF594 | 200 µg/ml | $364.00 | |||
megsin Antibody (H-10) Alexa Fluor® 647 | sc-515694 AF647 | 200 µg/ml | $364.00 | |||
megsin Antibody (H-10) Alexa Fluor® 680 | sc-515694 AF680 | 200 µg/ml | $364.00 | |||
megsin Antibody (H-10) Alexa Fluor® 790 | sc-515694 AF790 | 200 µg/ml | $364.00 |