ME1 Antibody (C-6): sc-365891

ME1 (C-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects ME1 protein of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). Anti-ME1 antibody (C-6) is available as both non-conjugated form, as well as multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. ME1 (Malic enzyme 1), also known as NADP-ME, MES or HUMNDME, is a 572 amino acid cytoplasmic protein that belongs to the malic enzyme family. Expressed ubiquitously with highest expression in liver and white adipose tissue, ME1 functions as an NADP-dependent enzyme that catalyzes the conversion of S-malate and NADP to pyruvate, carbon dioxide and NADPH (a reducing agent that participates in fatty acid biosynthesis). Through ME1′s ability to catalyze the reversible oxidative decarboxylation of malate, ME1 links the citric acid and glycolytic cycles. ME1 exists as a homotetramer that uses divalent metal cations, such as magnesium or manganese, as cofactors. ME1 expression is regulated by both thyroid hormone levels and the amount of carbohydrates in the diet, indicating that ME1 may play an important role as a housekeeping protein within the cell.

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ME1 Antibody (C-6) References:

1. Enhanced gastrointestinal expression of cytosolic malic enzyme (ME1) induces intestinal and liver lipogenic gene expression and intestinal cell proliferation in mice.  |  Al-Dwairi, A., et al. 2014. PLoS One. 9: e113058. PMID: 25402228
2. Malic Enzyme 1 (ME1) is pro-oncogenic in ApcMin/+ mice.  |  Fernandes, LM., et al. 2018. Sci Rep. 8: 14268. PMID: 30250042
3. ME1 promotes basal-like breast cancer progression and associates with poor prognosis.  |  Liao, R., et al. 2018. Sci Rep. 8: 16743. PMID: 30425310
4. Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis.  |  Shao, C., et al. 2020. Redox Biol. 36: 101685. PMID: 32853879
5. Fine mapping identifies NAD-ME1 as a candidate underlying a major locus controlling temporal variation in primary and specialized metabolism in Arabidopsis.  |  Francisco, M., et al. 2021. Plant J. 106: 454-467. PMID: 33523525
6. CircME1 promotes aerobic glycolysis and sunitinib resistance of clear cell renal cell carcinoma through cis-regulation of ME1.  |  Zhang, MX., et al. 2022. Oncogene. 41: 3979-3990. PMID: 35798876
7. High ME1 Expression Is a Molecular Predictor of Post-Transplant Survival of Patients with Acute Myeloid Leukemia.  |  Ortiz Rojas, CA., et al. 2022. Cancers (Basel). 15: PMID: 36612292
8. Malic Enzyme 1 (ME1) Promotes Adiposity and Hepatic Steatosis and Induces Circulating Insulin and Leptin in Obese Female Mice.  |  Simmen, FA., et al. 2023. Int J Mol Sci. 24: PMID: 37047583
9. Compensatory activity of the PC-ME1 metabolic axis underlies differential sensitivity to mitochondrial complex I inhibition.  |  Del Prado, L., et al. 2024. Nat Commun. 15: 8682. PMID: 39375345
10. A novel initiator regulates expression of the nontissue-specific helix-loop-helix gene ME1.  |  Shain, DH., et al. 1995. Nucleic Acids Res. 23: 1696-703. PMID: 7784173

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ME1 Antibody (C-6) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-ME1 Antibody (C-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ME1 Antibody (C-6) Specifications

Alternate Product Name: ME1

Product Clone Name: C-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 34-80

Epitope Target: ME1

Epitope Species: human

Product Reactivity/Detection: ME1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for ME1 Antibody (C-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred ME1 antibody is ME1 Antibody (99.1): sc-100569.