Anti-MCP-1 Antibody (5D3-F7) recommended for detection of MCP-1 of human origin by WB, IP and IF

Anti-MCP-1 Antibody (5D3-F7): sc-32786

Anti-MCP-1 Antibody (5D3-F7) is rated 3.0 out of 5 by 1.
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Datasheets

    • MCP-1 Antibody (5D3-F7) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant MCP-1 of human origin
    • recommended for detection of MCP-1 of human origin by WB, IP and IF
    • See MCP-1-4/eotaxin (B-2): sc-377082 for MCP-1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MCP-1 Antibody (5D3-F7) for WB applications. These reagents are now offered in bundles with MCP-1 Antibody (5D3-F7) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-32786: MCP-1 (5D3-F7) monoclonal antibody?

Asked by: Professor Griffin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 3 out of 5 by from Band detected in WB using human recombinant MCP Band detected in WB using human recombinant MCP-1. -SCBT QC
Date published: 2013-02-14
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