MCP-1-4/eotaxin Antibody (B-2) recommended for detection of MCP-1, MCP-2, MCP-3, MCP-4 and eotaxin of human origin by WB, IP, IF, IHC(P) and ELISA

MCP-1-4/eotaxin Antibody (B-2): sc-377082

MCP-1-4/eotaxin Antibody (B-2) is rated 4.0 out of 5 by 6.
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Datasheets

    • Anti-MCP-1-4/eotaxin Antibody (B-2) is a mouse monoclonal IgG1 (kappa light chain) MCP-1-4/eotaxin antibody provided at 200 µg/ml
    • raised against amino acids 1-99 representing full length MCP-1 of human origin
    • MCP-1-4/eotaxin Antibody (B-2) is recommended for detection of MCP-1, MCP-2, MCP-3, MCP-4 and eotaxin of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-MCP-1-4/eotaxin Antibody (B-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MCP-1-4/eotaxin (B-2): sc-377082.

Can MCP-1-4/eotaxin (B-2): sc-377082 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, MCP-1-4/eotaxin (B-2): sc-377082 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from okay antibody I tried this antibody in the ratio 1:1000 to detect CCL2 in NHBE whole cell lysate and luckily we got a band around 12kDa for the first time. But after that we tried in Beas2b cell lysate and again in NHBE lysate too but we couldn't see the band. Maybe it will work only in freshly prepared antibody so its not a user-friendly.
Date published: 2017-03-14
Rated 5 out of 5 by from only for WB we do human samples stimulated by IL1B, and we see spicific band at 11kD, and also there is a none spicific band at 40kD. But we do not consider it could do IHC-P.
Date published: 2016-12-14
Rated 2 out of 5 by from Robust band seen in WB using human lung tissue Robust band seen in WB using human lung tissue extract. -SCBT QC
Date published: 2015-04-20
Rated 4 out of 5 by from Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Date published: 2015-02-03
Rated 5 out of 5 by from Published WB and IHC data with mouse samples Published WB and IHC data with mouse samples - SCBT Publication Review
Date published: 2015-01-19
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human upper stomach tissue. -SCBT QC
Date published: 2013-01-18
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