MCP-1 Antibody (5J): sc-32771

MCP-1 Antibody (5J) is a mouse monoclonal IgG1 kappa light chain antibody that detects MCP-1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-MCP-1 antibody (5J) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MCP-1, also known as CCL2 or C-C motif chemokine ligand 2, plays a crucial role in the immune response by mediating the recruitment of monocytes to sites of inflammation, which is vital for effective immune surveillance and tissue repair. Elevated levels of MCP-1 are observed during infection and inflammatory conditions, highlighting MCP-1′s importance in leukocyte infiltration and the overall inflammatory response. MCP-1 interacts with two distinct receptors, which differ in their carboxy-termini, allowing for diverse signaling pathways that can influence various immune cell behaviors. Understanding MCP-1′s function and interactions is essential for elucidating its role in both normal physiology and pathological conditions, making MCP-1 (5J) monoclonal antibody a valuable tool for researchers studying immune responses and inflammatory diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MCP-1 Antibody (5J) References:

1. MCP-1/CCR2 axis inhibition sensitizes the brain microenvironment against melanoma brain metastasis progression.  |  Pozzi, S., et al. 2022. JCI Insight. 7: PMID: 35980743
2. Notch-mediated hepatocyte MCP-1 secretion causes liver fibrosis.  |  Kang, J., et al. 2023. JCI Insight. 8: PMID: 36752206
3. Role of MCP-1/CCR2 axis in renal fibrosis: Mechanisms and therapeutic targeting.  |  He, S., et al. 2023. Medicine (Baltimore). 102: e35613. PMID: 37861543
4. Role of MCP-1 as an inflammatory biomarker in nephropathy.  |  Liu, Y., et al. 2023. Front Immunol. 14: 1303076. PMID: 38239353
5. Monocyte chemotactic protein MCP-2 activates human basophil and eosinophil leukocytes similar to MCP-3.  |  Weber, M., et al. 1995. J Immunol. 154: 4166-72. PMID: 7535823
6. Monocyte chemotactic protein-1 (MCP-1), -2, and -3 are chemotactic for human T lymphocytes.  |  Taub, DD., et al. 1995. J Clin Invest. 95: 1370-6. PMID: 7883984
7. Molecular cloning and functional expression of two monocyte chemoattractant protein 1 receptors reveals alternative splicing of the carboxyl-terminal tails.  |  Charo, IF., et al. 1994. Proc Natl Acad Sci U S A. 91: 2752-6. PMID: 8146186
8. Monocyte chemoattractant protein-3 is a functional ligand for CC chemokine receptors 1 and 2B.  |  Combadiere, C., et al. 1995. J Biol Chem. 270: 29671-5. PMID: 8530354
9. Human monocyte chemotactic proteins-2 and -3: structural and functional comparison with MCP-1.  |  Proost, P., et al. 1996. J Leukoc Biol. 59: 67-74. PMID: 8558070
10. Chemokines in seasonal allergic rhinitis.  |  Kuna, P., et al. 1996. J Allergy Clin Immunol. 97: 104-12. PMID: 8568122
11. Early signal transduction by the receptor to the chemokine monocyte chemotactic protein-1 in a murine T cell hybrid.  |  Dubois, PM., et al. 1996. J Immunol. 156: 1356-61. PMID: 8568234
12. Site-directed mutagenesis of monocyte chemoattractant protein-1 identifies two regions of the polypeptide essential for biological activity.  |  Beall, CJ., et al. 1996. Biochem J. 313 (Pt 2): 633-40. PMID: 8573103

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MCP-1 Antibody (5J) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-MCP-1 Antibody (5J) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MCP-1 Antibody (5J) Specifications

Alternate Product Name: CCL2

Product Clone Name: 5J

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: MCP-1

Epitope Species: human

Product Reactivity/Detection: MCP-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for MCP-1 Antibody (5J): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, L: Azide-Free, PE: Phycoerythrin