MCM5 Antibody (G-1): sc-165995

MCM5 Antibody (G-1) is a mouse monoclonal IgG2a antibody that detects MCM5 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MCM5 plays a crucial role in DNA replication as a member of the mini-chromosome maintenance (MCM) protein family, which is essential for ensuring that DNA replication occurs only once per cell cycle. This regulation is vital for maintaining genomic stability, as improper replication can lead to mutations and cancer. MCM5, along with other MCM proteins, is involved in the formation of the MCM2-7 complex, which acts as a helicase to unwind DNA during replication. MCM5 is particularly important during the transition from the G1 to S phase of the cell cycle, where expression peaks, indicating an active role in preparing the cell for DNA synthesis. Additionally, MCM5 has been implicated in the activation of transcription factors such as Stat1α in response to interferon-gamma, linking MCM5 to cellular signaling pathways that influence immune responses. Anti-MCM5 antibody (G-1) is an invaluable tool for researchers studying cell cycle regulation, DNA replication mechanisms, and the molecular underpinnings of various diseases.

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MCM5 Antibody (G-1) References:

1. Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-gamma.  |  DaFonseca, CJ., et al. 2001. Proc Natl Acad Sci U S A. 98: 3034-9. PMID: 11248027
2. CDC46/MCM5, a yeast protein whose subcellular localization is cell cycle-regulated, is involved in DNA replication at autonomously replicating sequences.  |  Chen, Y., et al. 1992. Proc Natl Acad Sci U S A. 89: 10459-63. PMID: 1438234
3. Prediction and diagnosis of bladder cancer recurrence based on urinary content of hTERT, SENP1, PPP1CA, and MCM5 transcripts.  |  Brems-Eskildsen, AS., et al. 2010. BMC Cancer. 10: 646. PMID: 21106093
4. Increased expression of MCM5 is significantly associated with aggressive progression and poor prognosis of oral squamous cell carcinoma.  |  Yu, SY., et al. 2014. J Oral Pathol Med. 43: 344-9. PMID: 24245508
5. MCM5: a new actor in the link between DNA replication and Meier-Gorlin syndrome.  |  Vetro, A., et al. 2017. Eur J Hum Genet. 25: 646-650. PMID: 28198391
6. Minichromosomal Maintenance Component Complex 5 (MCM5) as a Marker of Barrett's Esophagus-Related Neoplasia: A Feasibility Study.  |  Everson, M., et al. 2019. Dig Dis Sci. 64: 2815-2822. PMID: 30982210
7. MCM5 Expression Is Associated With the Grade of Malignancy and Ki-67 Antigen in LSCC.  |  Nowinska, K., et al. 2019. Anticancer Res. 39: 2325-2335. PMID: 31092424
8. Predictive value of MCM5 (ADXBLADDER) analysis in urine of men evaluated for the initial diagnosis of bladder cancer: A comparative prospective study.  |  Anastasi, E., et al. 2020. Diagn Cytopathol. 48: 1034-1040. PMID: 32562513
9. The novel peptide PFAP1 promotes primordial follicle activation by binding to MCM5.  |  Zhang, Y., et al. 2023. FASEB J. 37: e22931. PMID: 37086099
10. MEG3 shuttled by exosomes released from human bone marrow mesenchymal stem cells promotes TP53 stability to regulate MCM5 transcription in keloid fibroblasts.  |  Zhu, F., et al. 2024. J Gene Med. 26: e3688. PMID: 38686583

The preferred MCM5 antibody is MCM5 Antibody (E-10): sc-165994.