MCM5 Antibody (E-10): sc-165994

MCM5 Antibody (E-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects MCM5 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-MCM5 antibody (E-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The mini-chromosome maintenance (MCM) family of proteins, which includes MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5), and MCM7 (Cdc47), plays a crucial role in regulating DNA replication, ensuring that replication occurs only once per cell cycle. MCM5 is essential for forming the MCM2-7 complex, which acts as a helicase during DNA unwinding, a critical step for DNA replication. Proper functioning of MCM5 maintains genomic stability, as any dysregulation can lead to uncontrolled cell proliferation and contribute to oncogenesis. MCM proteins are tightly regulated by E2F transcription factors and various protein kinases, which help maintain the post-replicative state of the cell. MCM5 is also involved in activating Stat1α transcription in response to interferon-gamma, highlighting its role in both cell cycle regulation and immune response.

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MCM5 Antibody (E-10) References:

1. MCM2 and MCM5 as prognostic markers in colon cancer: a worthwhile approach.  |  Burger, M. 2009. Dig Dis Sci. 54: 197-8. PMID: 18716878
2. The cyclin A centrosomal localization sequence recruits MCM5 and Orc1 to regulate centrosome reduplication.  |  Ferguson, RL., et al. 2010. J Cell Sci. 123: 2743-9. PMID: 20663915
3. The role of MCM5 expression in cervical cancer: Correlation with progression and prognosis.  |  Wang, D., et al. 2018. Biomed Pharmacother. 98: 165-172. PMID: 29253764
4. Detection of MCM5 as a novel non-invasive aid for the diagnosis of endometrial and ovarian tumours.  |  Stockley, J., et al. 2020. BMC Cancer. 20: 1000. PMID: 33059604
5. A common set of conserved motifs in a vast variety of putative nucleic acid-dependent ATPases including MCM proteins involved in the initiation of eukaryotic DNA replication.  |  Koonin, EV. 1993. Nucleic Acids Res. 21: 2541-7. PMID: 8332451
6. A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex.  |  Ishimi, Y. 1997. J Biol Chem. 272: 24508-13. PMID: 9305914
7. Protein kinase inhibition in G2 causes mammalian Mcm proteins to reassociate with chromatin and restores ability to replicate.  |  Coverley, D., et al. 1998. Exp Cell Res. 238: 63-9. PMID: 9457057
8. Selective proteolysis of the nuclear replication factor MCM3 in apoptosis.  |  Schwab, BL., et al. 1998. Exp Cell Res. 238: 415-21. PMID: 9473350
9. Cell cycle- and chromatin binding state-dependent phosphorylation of human MCM heterohexameric complexes. A role for cdc2 kinase.  |  Fujita, M., et al. 1998. J Biol Chem. 273: 17095-101. PMID: 9642275
10. E2F3 activity is regulated during the cell cycle and is required for the induction of S phase.  |  Leone, G., et al. 1998. Genes Dev. 12: 2120-30. PMID: 9679057
11. Ser727-dependent recruitment of MCM5 by Stat1alpha in IFN-gamma-induced transcriptional activation.  |  Zhang, JJ., et al. 1998. EMBO J. 17: 6963-71. PMID: 9843502
12. Human T-cell leukemia virus type 1 tax protein abrogates interleukin-2 dependence in a mouse T-cell line.  |  Iwanaga, Y., et al. 1999. J Virol. 73: 1271-7. PMID: 9882331

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MCM5 Antibody (E-10) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-MCM5 Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MCM5 Antibody (E-10) Specifications

Alternate Product Name: MCM5

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: MCM5

Epitope Species: human

Product Reactivity/Detection: MCM5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for MCM5 Antibody (E-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin