Anti-MCM5 Antibody (E-10) recommended for detection of MCM5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-MCM5 Antibody (E-10): sc-165994

Anti-MCM5 Antibody (E-10) is rated 4.9 out of 5 by 9.
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Datasheets

    • Anti-MCM5 Antibody (E-10) is a mouse monoclonal IgG2b κ MCM5 antibody, cited in 9 publications, provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of MCM5 of human origin
    • Anti-MCM5 Antibody (E-10) is recommended for detection of MCM5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-MCM5 Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MCM5 (E-10): sc-165994.
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MCM5 Antibody (E-10) for WB and IHC(P) applications. These reagents are now offered in bundles with MCM5 Antibody (E-10) (see ordering information below).
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I am using MCM5 (E-10): sc-165994 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Western blot One strong band around 90KDa in MNase lysate of mESC
Date published: 2018-03-26
Rated 5 out of 5 by from good features 基 本没 有杂 带,牛奶 封闭,一抗过夜,二抗牛奶室温两小时,30ug细胞蛋白,跑出条带如下 图所示
Date published: 2018-03-11
Rated 5 out of 5 by from a good antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from Excellent for western Very clean and strong band with 1:1,000 dilution for mouse tissue lysates
Date published: 2017-08-04
Rated 5 out of 5 by from Great antibody MCM5 Antibody was used in WB with 1:1000 dilution, the band is strong and specific.
Date published: 2017-04-08
Rated 5 out of 5 by from excellent antibody I used this antibody for IP (4ug ab for 700ug of protein) and WB (1:1000). Worked beautifully for both of the experiments
Date published: 2017-02-06
Rated 5 out of 5 by from Superb immunoperoxidase nuclear staining Superb immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date published: 2015-04-26
Rated 5 out of 5 by from Positive results in western blot analysis Positive results in western blot analysis of MCM5 expression in non-transfected and human MCM5 transfected 293 whole cell lysates a;so in HL-60 and KNRK nuclear extracts. -SCBT QC
Date published: 2015-02-24
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