Anti-MCM2 Antibody (E-8) recommended for detection of MCM2 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-MCM2 Antibody (E-8): sc-373702

Anti-MCM2 Antibody (E-8) is rated 4.5 out of 5 by 13.
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Datasheets

    • Anti-MCM2 Antibody (E-8) is a mouse monoclonal IgG2b κ MCM2 antibody, cited in 13 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 9-34 at the N-terminus of MCM2 of human origin
    • Anti-MCM2 Antibody (E-8) is recommended for detection of MCM2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-MCM2 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MCM2 (E-8): sc-373702.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MCM2 Antibody (E-8) for WB applications. These reagents are now offered in bundles with MCM2 Antibody (E-8) (see ordering information below).
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What application is the blocking peptide sc-373702 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good antibody Antibody signal is good and clear upregulation is seen after x-ray irradiation, as expected.
Date published: 2021-07-28
Rated 5 out of 5 by from Stains specifically Steins specifically, colocalizes well with the nucleus. Minor cytoplasm staining detected. Verified specificity with blocking peptide and non dividing resting cells. 647 pairs well with 594 costaining with two different ABs. The typical bleedthrough of fluorescence 647 to 594 is 0, and 594 to 647 channel is only 5-10%. Excellent AB overall.
Date published: 2019-04-15
Rated 3 out of 5 by from MCM2 Ab Antibody works for WB. But signal is quite weak. Protein was weakly detected at dilution 1:100 using HCT116 whole cell lysates. Band corresponding to MCM2 protein was observed around 110-120kDa.
Date published: 2018-07-23
Rated 5 out of 5 by from good features good 基本没有杂带,牛奶封闭,一抗过夜,二抗牛奶室温两小时,30ug细胞蛋白,跑出条带如下图所示
Date published: 2018-03-11
Rated 5 out of 5 by from Works well for western blot Works well for western of whole cell extracts at 1:1000 in 5% milk
Date published: 2018-01-16
Rated 5 out of 5 by from a fantastic antibody for WB This antibody generates a clear and strong signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from Good antibody! We got specific signal for MCM2 @ about 130kd in WB test.
Date published: 2017-08-22
Rated 5 out of 5 by from High specificity 24hpf,2dpf,3dpf zebrefish embryos, Western blot, A clear single target band,indicates that MCM2 Antibody (E-8) the antibody with high specificity.
Date published: 2017-04-17
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