MCM2 Antibody (E-8): sc-373702

MCM2 Antibody (E-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects MCM2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MCM2 (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The mini-chromosome maintenance (MCM) family of proteins, which includes MCM2, plays a crucial role in the regulation of DNA replication, ensuring that DNA is replicated only once during each cell cycle. MCM2 is particularly important as MCM2 forms part of the MCM2-7 complex, which acts as a helicase to unwind DNA, a critical step for the initiation of DNA replication. This unwinding is essential for proper genome duplication, and any dysregulation can lead to genomic instability, a hallmark of many cancers. MCM2 expression increases during cell growth, peaking at the transition from G1 to S phase of the cell cycle, and is tightly regulated by E2F transcription factors and various protein kinases. Additionally, MCM2/MCM4 complexes serve as substrates for Cdc2/cyclin B, highlighting MCM2′s role in cell cycle regulation. MCM3 cleavage during apoptosis, which can be inhibited by caspase inhibitors, leads to MCM complex inactivation, further emphasizing MCM proteins′ importance in maintaining cellular integrity and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

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MCM2 Antibody (E-8) References:

1. The ORC/Cdc6/MCM2–7 complex, a new power player for regulated helicase loading.  |  Riera, A., et al. 2013. Cell Cycle. 12: 2155-6. PMID: 23803736
2. Changes in MCM2-7 proteins at senescence.  |  Suzuki, Y., et al. 2019. Genes Genet Syst. 94: 123-132. PMID: 31092751
3. MCM2 promotes the stemness of endometrial cancer cells via the Akt/β-catenin pathway.  |  Chu, Y., et al. 2023. Neoplasma. 70: 610-620. PMID: 38053378
4. Assembly, Activation, and Helicase Actions of MCM2-7: Transition from Inactive MCM2-7 Double Hexamers to Active Replication Forks.  |  You, Z. and Masai, H. 2024. Biology (Basel). 13: PMID: 39194567
5. A common set of conserved motifs in a vast variety of putative nucleic acid-dependent ATPases including MCM proteins involved in the initiation of eukaryotic DNA replication.  |  Koonin, EV. 1993. Nucleic Acids Res. 21: 2541-7. PMID: 8332451
6. A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex.  |  Ishimi, Y. 1997. J Biol Chem. 272: 24508-13. PMID: 9305914
7. Protein kinase inhibition in G2 causes mammalian Mcm proteins to reassociate with chromatin and restores ability to replicate.  |  Coverley, D., et al. 1998. Exp Cell Res. 238: 63-9. PMID: 9457057
8. Selective proteolysis of the nuclear replication factor MCM3 in apoptosis.  |  Schwab, BL., et al. 1998. Exp Cell Res. 238: 415-21. PMID: 9473350
9. Cell cycle- and chromatin binding state-dependent phosphorylation of human MCM heterohexameric complexes. A role for cdc2 kinase.  |  Fujita, M., et al. 1998. J Biol Chem. 273: 17095-101. PMID: 9642275
10. E2F3 activity is regulated during the cell cycle and is required for the induction of S phase.  |  Leone, G., et al. 1998. Genes Dev. 12: 2120-30. PMID: 9679057
11. Ser727-dependent recruitment of MCM5 by Stat1alpha in IFN-gamma-induced transcriptional activation.  |  Zhang, JJ., et al. 1998. EMBO J. 17: 6963-71. PMID: 9843502
12. Human T-cell leukemia virus type 1 tax protein abrogates interleukin-2 dependence in a mouse T-cell line.  |  Iwanaga, Y., et al. 1999. J Virol. 73: 1271-7. PMID: 9882331

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MCM2 Antibody (E-8) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-MCM2 Antibody (E-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MCM2 Antibody (E-8) Specifications

Alternate Product Name: MCM2

Product Clone Name: E-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 9-34

Epitope Target: MCM2

Epitope Species: human

Product Reactivity/Detection: MCM2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for MCM2 Antibody (E-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin