MBNL2 Antibody (3B4): sc-136167

MBNL2 Antibody (3B4) is a mouse monoclonal IgG2b kappa light chain antibody that detects MBNL2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-MBNL2 antibody (3B4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MBNL2, a 367 amino acid protein, plays a crucial role in regulating pre-mRNA splicing, essential for proper gene expression. MBNL2 interacts with expanded CUG repeats, particularly in myotonic dystrophy types 1 and 2, where MBNL2 localizes to nuclear foci, suggesting a pathogenic role in these disorders. MBNL2 is involved in recruiting integrin α3 to focal adhesions through an RNA-dependent protein localization mechanism, highlighting MBNL2′s significance in cellular adhesion and signaling processes. MBNL2′s ability to modulate splicing and interactions with other proteins underscores MBNL2′s importance in maintaining cellular function and potential implications in disease mechanisms.

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MBNL2 Antibody (3B4) References:

1. Recruitment of human muscleblind proteins to (CUG)(n) expansions associated with myotonic dystrophy.  |  Miller, JW., et al. 2000. EMBO J. 19: 4439-48. PMID: 10970838
2. Muscleblind localizes to nuclear foci of aberrant RNA in myotonic dystrophy types 1 and 2.  |  Mankodi, A., et al. 2001. Hum Mol Genet. 10: 2165-70. PMID: 11590133
3. Three proteins, MBNL, MBLL and MBXL, co-localize in vivo with nuclear foci of expanded-repeat transcripts in DM1 and DM2 cells.  |  Fardaei, M., et al. 2002. Hum Mol Genet. 11: 805-14. PMID: 11929853
4. MBNL1 is the primary determinant of focus formation and aberrant insulin receptor splicing in DM1.  |  Dansithong, W., et al. 2005. J Biol Chem. 280: 5773-80. PMID: 15546872
5. [Molecular pathways to myotonic dystrophy].  |  Ishiura, S. 2005. Nihon Rinsho. 63: 515-21. PMID: 15773354
6. Dynamic balance between activation and repression regulates pre-mRNA alternative splicing during heart development.  |  Ladd, AN., et al. 2005. Dev Dyn. 233: 783-93. PMID: 15830352
7. Colocalization of muscleblind with RNA foci is separable from mis-regulation of alternative splicing in myotonic dystrophy.  |  Ho, TH., et al. 2005. J Cell Sci. 118: 2923-33. PMID: 15961406
8. The Muscleblind family of proteins: an emerging class of regulators of developmentally programmed alternative splicing.  |  Pascual, M., et al. 2006. Differentiation. 74: 65-80. PMID: 16533306
9. Calpain-2 Mediates MBNL2 Degradation and a Developmental RNA Processing Program in Neurodegeneration.  |  Wang, LH., et al. 2022. J Neurosci. 42: 5102-5114. PMID: 35606145
10. Studying the Effect of MBNL1 and MBNL2 Loss in Skeletal Muscle Regeneration.  |  Yadava, RS., et al. 2024. Int J Mol Sci. 25: PMID: 38473933
11. MBNL2 promotes aging-related cardiac fibrosis via inhibited SUMOylation of Krüppel-like factor4.  |  Lu, J., et al. 2024. iScience. 27: 110163. PMID: 38974966
12. Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro.  |  Ishikawa, K., et al. 1997. DNA Res. 4: 307-13. PMID: 9455477

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MBNL2 Antibody (3B4) has 27 citations in a variety of scientific publications.

Primary antibodies like Anti-MBNL2 Antibody (3B4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MBNL2 Antibody (3B4) Specifications

Alternate Product Name: MBNL2

Product Clone Name: 3B4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: MBNL2

Epitope Species: human

Product Reactivity/Detection: MBNL2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 27 citations in various scientific publications

Available Conjugates/Forms for MBNL2 Antibody (3B4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin