MBNL1/2 Antibody (5F9): sc-136166

MBNL1/2 Antibody (5F9) is a mouse monoclonal IgG1 kappa light chain antibody that detects MBNL1/2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-MBNL1/2 antibody (5F9) is available as the non-conjugated format. MBNL1, consisting of 370 amino acids, plays a crucial role in regulating pre-mRNA splicing, a vital process for gene expression. MBNL1 is predominantly expressed in various tissues, including skeletal muscle, prostate, lung, heart, small intestine, ovary, and placenta, highlighting importance in both muscle and organ function. MBNL1 and MBNL2 interact with expanded CUG repeats, implicated in myotonic dystrophy types 1 and 2 (DM1 and DM2). MBNL1/2 (5F9) monoclonal antibody localizes to nuclear foci in affected tissues, suggesting that accumulation of mutant RNA is pathogenic in these disorders. By antagonizing CUG-BP and ETR-3-like factors, MBNL1 and MBNL2 facilitate inclusion or exclusion of specific exons in pre-mRNAs, influencing splicing process and ultimately affecting gene expression. MBNL1/2 (5F9) antibody detection across multiple species makes this reagent invaluable for researchers studying molecular mechanisms underlying myotonic dystrophies and related conditions.

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MBNL1/2 Antibody (5F9) References:

1. Developmental expression of mouse muscleblind genes Mbnl1, Mbnl2 and Mbnl3.  |  Kanadia, RN., et al. 2003. Gene Expr Patterns. 3: 459-62. PMID: 12915312
2. Tau exon 2 responsive elements deregulated in myotonic dystrophy type I are proximal to exon 2 and synergistically regulated by MBNL1 and MBNL2.  |  Carpentier, C., et al. 2014. Biochim Biophys Acta. 1842: 654-64. PMID: 24440524
3. Opposing roles of miR-294 and MBNL1/2 in shaping the gene regulatory network of embryonic stem cells.  |  Wu, DR., et al. 2018. EMBO Rep. 19: PMID: 29735517
4. (CTG)n repeat-mediated dysregulation of MBNL1 and MBNL2 expression during myogenesis in DM1 occurs already at the myoblast stage.  |  André, LM., et al. 2019. PLoS One. 14: e0217317. PMID: 31116797
5. Mbnl1 and Mbnl2 regulate brain structural integrity in mice.  |  Sta Maria, NS., et al. 2021. Commun Biol. 4: 1342. PMID: 34848815
6. MBNL1 drives dynamic transitions between fibroblasts and myofibroblasts in cardiac wound healing.  |  Bugg, D., et al. 2022. Cell Stem Cell. 29: 419-433.e10. PMID: 35176223
7. Mice lacking MBNL1 and MBNL2 exhibit sudden cardiac death and molecular signatures recapitulating myotonic dystrophy.  |  Lee, KY., et al. 2022. Hum Mol Genet. 31: 3144-3160. PMID: 35567413
8. Alternative splicing mediates the compensatory upregulation of MBNL2 upon MBNL1 loss-of-function.  |  Nitschke, L., et al. 2023. Nucleic Acids Res. 51: 1245-1259. PMID: 36617982
9. MBNL1 Regulates Programmed Postnatal Switching Between Regenerative and Differentiated Cardiac States.  |  Bailey, LRJ., et al. 2024. Circulation. 149: 1812-1829. PMID: 38426339
10. Studying the Effect of MBNL1 and MBNL2 Loss in Skeletal Muscle Regeneration.  |  Yadava, RS., et al. 2024. Int J Mol Sci. 25: PMID: 38473933

The preferred MBNL2 antibody is MBNL2 Antibody (3B4): sc-136167.