MBNL1 Antibody (3A4): sc-47740

MBNL1 Antibody (3A4) is a mouse monoclonal IgG1 kappa light chain antibody that detects MBNL1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-MBNL1 antibody (3A4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MBNL1, consisting of 370 amino acids, plays a crucial role in regulating pre-mRNA splicing, a vital process for gene expression. MBNL1 is predominantly expressed in tissues such as skeletal muscle, prostate, lung, heart, small intestine, ovary, and placenta. MBNL1, along with homologs MBNL2 and MBNL3, modulates specific exons in pre-mRNAs by counteracting the activity of CUG-BP and ETR-3-like factors, which bind to distinct intronic sites. This regulation is particularly important in myotonic dystrophy types 1 and 2, where MBNL1 and MBNL2 localize to nuclear foci associated with expanded CUG repeats, suggesting that accumulation of mutant RNA in the nucleus contributes to the pathogenesis of these disorders. MBNL1′s interaction with RNA elements highlights its significance in maintaining proper cellular function and gene expression, making MBNL1 (3A4) monoclonal antibody an essential tool for researchers studying splicing mechanisms and related diseases.

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MBNL1 Antibody (3A4) References:

1. Recruitment of human muscleblind proteins to (CUG)(n) expansions associated with myotonic dystrophy.  |  Miller, JW., et al. 2000. EMBO J. 19: 4439-48. PMID: 10970838
2. Muscleblind localizes to nuclear foci of aberrant RNA in myotonic dystrophy types 1 and 2.  |  Mankodi, A., et al. 2001. Hum Mol Genet. 10: 2165-70. PMID: 11590133
3. Three proteins, MBNL, MBLL and MBXL, co-localize in vivo with nuclear foci of expanded-repeat transcripts in DM1 and DM2 cells.  |  Fardaei, M., et al. 2002. Hum Mol Genet. 11: 805-14. PMID: 11929853
4. MBNL1 is the primary determinant of focus formation and aberrant insulin receptor splicing in DM1.  |  Dansithong, W., et al. 2005. J Biol Chem. 280: 5773-80. PMID: 15546872
5. [Molecular pathways to myotonic dystrophy].  |  Ishiura, S. 2005. Nihon Rinsho. 63: 515-21. PMID: 15773354
6. Dynamic balance between activation and repression regulates pre-mRNA alternative splicing during heart development.  |  Ladd, AN., et al. 2005. Dev Dyn. 233: 783-93. PMID: 15830352
7. Colocalization of muscleblind with RNA foci is separable from mis-regulation of alternative splicing in myotonic dystrophy.  |  Ho, TH., et al. 2005. J Cell Sci. 118: 2923-33. PMID: 15961406
8. The Muscleblind family of proteins: an emerging class of regulators of developmentally programmed alternative splicing.  |  Pascual, M., et al. 2006. Differentiation. 74: 65-80. PMID: 16533306
9. The Regulatory Role of Both MBNL1 and MBNL1-AS1 in Several Common Cancers.  |  Zhang, Q., et al. 2022. Curr Pharm Des. 28: 581-585. PMID: 34459372
10. MBNL1 drives dynamic transitions between fibroblasts and myofibroblasts in cardiac wound healing.  |  Bugg, D., et al. 2022. Cell Stem Cell. 29: 419-433.e10. PMID: 35176223
11. MBNL1 Regulates Programmed Postnatal Switching Between Regenerative and Differentiated Cardiac States.  |  Bailey, LRJ., et al. 2024. Circulation. 149: 1812-1829. PMID: 38426339
12. Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro.  |  Ishikawa, K., et al. 1997. DNA Res. 4: 307-13. PMID: 9455477

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MBNL1 Antibody (3A4) has 32 citations in a variety of scientific publications.

Primary antibodies like Anti-MBNL1 Antibody (3A4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MBNL1 Antibody (3A4) Specifications

Alternate Product Name: MBNL1

Product Clone Name: 3A4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: MBNL1

Epitope Species: human

Product Reactivity/Detection: MBNL1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 32 citations in various scientific publications

Available Conjugates/Forms for MBNL1 Antibody (3A4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin