MBD4 Antibody (G-7): sc-398249
- MBD4 Antibody (G-7) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 521-546 near the C-terminus of MBD4 of mouse origin
- recommended for detection of MBD4 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and bovine
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MBD4 Antibody (G-7) for WB applications. These reagents are now offered in bundles with MBD4 Antibody (G-7) (see ordering information below).
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MBD4 Antibody (G-7) is a mouse monoclonal IgG1 antibody that detects MBD4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MBD4, also known as MED1, plays a crucial role in the DNA damage repair process by interacting with the mismatch repair protein MLH1, which is essential for maintaining genomic stability. This interaction allows MBD4 to preferentially bind to methylated cytosine residues in mismatched base pairs, thereby facilitating the repair of DNA errors that could lead to mutations and potentially contribute to cancer development. The presence of MBD4 in somatic tissues underscores its importance in cellular processes, particularly in the context of epigenetic regulation and transcriptional repression associated with DNA methylation. Anti-MBD4 antibody (G-7) is an invaluable tool for researchers studying the intricate mechanisms of gene regulation and DNA repair pathways, providing insights into the molecular underpinnings of various diseases.
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MBD4 Antibody (G-7) References:
- Mbd4 inactivation increases Cright-arrowT transition mutations and promotes gastrointestinal tumor formation. | Wong, E., et al. 2002. Proc Natl Acad Sci U S A. 99: 14937-42. PMID: 12417741
- MBD4 mutations are rare in gastric carcinomas with microsatellite instability. | Pinto, M., et al. 2003. Cancer Genet Cytogenet. 145: 103-7. PMID: 12935920
- MBD4 deficiency reduces the apoptotic response to DNA-damaging agents in the murine small intestine. | Sansom, OJ., et al. 2003. Oncogene. 22: 7130-6. PMID: 14562041
- MBD4 cooperates with DNMT1 to mediate methyl-DNA repression and protects mammalian cells from oxidative stress. | Laget, S., et al. 2014. Epigenetics. 9: 546-56. PMID: 24434851
- MBD4 interacts with and recruits USP7 to heterochromatic foci. | Meng, H., et al. 2015. J Cell Biochem. 116: 476-85. PMID: 25358258
- DNMT1 cooperates with MBD4 to inhibit the expression of Glucocorticoid-induced TNFR-related protein in human T cells. | Wang, S., et al. 2017. FEBS Lett. 591: 1929-1939. PMID: 28542810
- MBD4 guards against methylation damage and germ line deficiency predisposes to clonal hematopoiesis and early-onset AML. | Sanders, MA., et al. 2018. Blood. 132: 1526-1534. PMID: 30049810
- Evolutionary Routes in Metastatic Uveal Melanomas Depend on MBD4 Alterations. | Rodrigues, M., et al. 2019. Clin Cancer Res. 25: 5513-5524. PMID: 31227496
- Methyl-CpG binding domain 4, DNA glycosylase (MBD4)-associated neoplasia syndrome associated with a homozygous missense variant in MBD4: Expansion of an emerging phenotype. | Blombery, P., et al. 2022. Br J Haematol. 198: 196-199. PMID: 35381620
- Cytidine analogs are synthetic lethal with base excision repair default due to MBD4 deficiency. | Chabot, T., et al. 2022. NPJ Precis Oncol. 6: 81. PMID: 36323843
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MBD4 Antibody (G-7) | sc-398249 | 200 µg/ml | $322.00 | |||
MBD4 Antibody (G-7): m-IgG Fc BP-HRP Bundle | sc-538405 | 200 µg Ab; 10 µg BP | $361.00 | |||
MBD4 Antibody (G-7): m-IgGκ BP-HRP Bundle | sc-536014 | 200 µg Ab; 40 µg BP | $361.00 | |||
MBD4 Antibody (G-7): m-IgG1 BP-HRP Bundle | sc-545686 | 200 µg Ab; 20 µg BP | $361.00 | |||
MBD4 (G-7) Neutralizing Peptide | sc-398249 P | 100 µg/0.5 ml | $69.00 |