Date published: 2026-5-5

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MAST205 Antibody (C-1): sc-514198

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Datasheets
  • MAST205 Antibody (C-1) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
  • raised against amino acids 1499-1798 mapping at the C-terminus of MAST205 of human origin
  • MAST205 Antibody (C-1) is recommended for detection of MAST205 of human origin by WB, IP, IF and ELISA
  • Anti-MAST205 Antibody (C-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MAST205 Antibody (C-1) for WB applications. These reagents are now offered in bundles with MAST205 Antibody (C-1) (see ordering information below).
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    MAST205 Antibody (C-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects MAST205 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAST205 Antibody (C-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MAST205, also known as microtubule-associated serine/threonine-protein kinase 2, is a testis-specific cytoplasmic protein that plays a crucial role in spermatid maturation by functioning within a multi-protein complex. MAST205 links the dystrophin/utrophin network with microtubule filaments through Syntrophin interaction, an adapter protein connecting signaling molecules to the dystrophin-associated protein complex. This complex maintains structural integrity of postsynaptic structures in both neuromuscular junction and central nervous system, influencing muscle function and signaling pathways. MAST205 interacts with TRAF6, regulating lipopolysaccharide-induced interleukin-12 synthesis in macrophages, significant for immune response modulation. MAST205 exists in two isoforms due to alternative splicing, with isoform 1 being full-length and isoform 2 lacking specific residues. N-terminus phosphorylation of MAST205 is required for ubiquitination, leading to proteasome-mediated proteolysis, highlighting the importance of post-translational modifications in regulating function and stability.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    MAST205 Antibody (C-1)

    sc-514198
    200 µg/ml
    $322.00

    MAST205 Antibody (C-1): m-IgG Fc BP-HRP Bundle

    sc-531062
    200 µg Ab; 10 µg BP
    $361.00

    MAST205 Antibody (C-1): m-IgGκ BP-HRP Bundle

    sc-524645
    200 µg Ab, 40 µg BP
    $361.00

    MAST205 Antibody (C-1): m-IgG1 BP-HRP Bundle

    sc-544379
    200 µg Ab; 20 µg BP
    $361.00

    MAST205 Antibody (C-1) AC

    sc-514198 AC
    500 µg/ml, 25% agarose
    $424.00

    MAST205 Antibody (C-1) HRP

    sc-514198 HRP
    200 µg/ml
    $322.00

    MAST205 Antibody (C-1) FITC

    sc-514198 FITC
    200 µg/ml
    $336.00

    MAST205 Antibody (C-1) PE

    sc-514198 PE
    200 µg/ml
    $349.00

    MAST205 Antibody (C-1) Alexa Fluor® 488

    sc-514198 AF488
    200 µg/ml
    $364.00

    MAST205 Antibody (C-1) Alexa Fluor® 546

    sc-514198 AF546
    200 µg/ml
    $364.00

    MAST205 Antibody (C-1) Alexa Fluor® 594

    sc-514198 AF594
    200 µg/ml
    $364.00

    MAST205 Antibody (C-1) Alexa Fluor® 647

    sc-514198 AF647
    200 µg/ml
    $364.00

    MAST205 Antibody (C-1) Alexa Fluor® 680

    sc-514198 AF680
    200 µg/ml
    $364.00

    MAST205 Antibody (C-1) Alexa Fluor® 790

    sc-514198 AF790
    200 µg/ml
    $364.00

    For Western Blot, is it recommended to use denatured or non-denatured conditions with MAST205 (C-1): sc-514198 monoclonal antibody?

    Asked by: Trav11
    Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
    Answered by: Technical Support
    Date published: 2017-02-27
    • y_2026, m_4, d_29, h_11CST
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    Rated 5 out of 5 by from Great Western blot data of MAST205 expression in KGreat Western blot data of MAST205 expression in K-562, HeLa and Jurkat whole cell lysates. -SCBT QC
    Date published: 2013-07-28
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    MAST205 Antibody (C-1) is rated 5.0 out of 5 by 1.
    • y_2026, m_4, d_29, h_11
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