MARCKS Antibody (JK-8): sc-100777

MARCKS Antibody (JK-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects MARCKS in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). MARCKS (JK-8) antibody is available in a non-conjugated form, providing flexibility for various experimental needs. Myristoylated alanine-rich protein kinase C substrate (MARCKS), also known as 80K or 80K-L, plays a crucial role as a major substrate for protein kinase C, which is vital for numerous cellular processes, including cell signaling and cytoskeletal organization. The human MARCKS protein consists of 332 amino acids and is primarily located at the plasma membrane, where MARCKS can rapidly dissociate upon phosphorylation by different protein kinase C isoforms, such as PKC α and PKC ε, which are particularly active in NIH/3T3 fibroblasts. This dynamic regulation of MARCKS is essential for its function in cellular signaling pathways. Additionally, MARCKS interacts with several important proteins, including Calmodulin, Actin, and Synapsin, and serves as a filamentous Actin crosslinking protein, highlighting MARCKS significance in maintaining cytoskeletal integrity and facilitating cellular communication.

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MARCKS Antibody (JK-8) References:

1. Relationship between the major protein kinase C substrates acidic 80-kDa protein-kinase-C substrate (80K) and myristoylated alanine-rich C-kinase substrate (MARCKS). Members of a gene family or equivalent genes in different species.  |  Herget, T., et al. 1992. Eur J Biochem. 209: 7-14. PMID: 1396720
2. Molecular cloning and chromosomal mapping of a cDNA encoding human 80K-L protein: major substrate for protein kinase C.  |  Sakai, K., et al. 1992. Genomics. 14: 175-8. PMID: 1427823
3. MARCKS is an actin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin.  |  Hartwig, JH., et al. 1992. Nature. 356: 618-22. PMID: 1560845
4. Molecular cloning, characterization, and expression of a cDNA encoding the '80- to 87-kDa' myristoylated alanine-rich C kinase substrate: a major cellular substrate for protein kinase C.  |  Stumpo, DJ., et al. 1989. Proc Natl Acad Sci U S A. 86: 4012-6. PMID: 2726763
5. Isolation of cDNAs encoding a substrate for protein kinase C: nucleotide sequence and chromosomal mapping of the gene for a human 80K protein.  |  Sakai, K., et al. 1989. Genomics. 5: 309-15. PMID: 2793184
6. MARCKS and MARCKS-like proteins in development and regeneration.  |  El Amri, M., et al. 2018. J Biomed Sci. 25: 43. PMID: 29788979
7. MARCKS as a Potential Therapeutic Target in Inflammatory Breast Cancer.  |  Manai, M., et al. 2022. Cells. 11: PMID: 36139501
8. The Role of MARCKS in Metastasis and Treatment Resistance of Solid Tumors.  |  Chiu, CL., et al. 2022. Cancers (Basel). 14: PMID: 36230850
9. Myristoylated, alanine-rich C-kinase substrate (MARCKS) regulates toll-like receptor 4 signaling in macrophages.  |  Issara-Amphorn, J., et al. 2023. Sci Rep. 13: 19562. PMID: 37949888
10. CircTRIM1 encodes TRIM1-269aa to promote chemoresistance and metastasis of TNBC via enhancing CaM-dependent MARCKS translocation and PI3K/AKT/mTOR activation.  |  Li, Y., et al. 2024. Mol Cancer. 23: 102. PMID: 38755678
11. Protein kinase C regulates MARCKS cycling between the plasma membrane and lysosomes in fibroblasts.  |  Allen, LA. and Aderem, A. 1995. EMBO J. 14: 1109-21. PMID: 7720702
12. Conventional PKC-alpha, novel PKC-epsilon and PKC-theta, but not atypical PKC-lambda are MARCKS kinases in intact NIH 3T3 fibroblasts.  |  Uberall, F., et al. 1997. J Biol Chem. 272: 4072-8. PMID: 9020116

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MARCKS Antibody (JK-8) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-MARCKS Antibody (JK-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MARCKS Antibody (JK-8) Specifications

Alternate Product Name: Myristoylated alanine-rich protein kinase C (PKC) substrate

Product Clone Name: JK-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2-66

Epitope Target: MARCKS

Epitope Species: human

Product Reactivity/Detection: MARCKS

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for MARCKS Antibody (JK-8): N/A

The preferred MARCKS antibody is MARCKS Antibody (C-6): sc-518073.