LOX Antibody (F-8) recommended for detection of Lysyl oxidase (LOX) of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

LOX Antibody (F-8): sc-373995

LOX Antibody (F-8) is rated 4.7 out of 5 by 7.
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Datasheets

    • LOX Antibody (F-8) is a mouse monoclonal IgG2a κ, cited in 12 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 160-193 within an internal region of LOX of human origin
    • Anti-LOX Antibody (F-8) is recommended for detection of Lysyl oxidase (LOX) of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine and porcine
    • Anti-LOX Antibody (F-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of LOX (F-8): sc-373995.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for LOX Antibody (F-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with LOX Antibody (F-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

I would like to know what is the negative control for LOX and LMO2 antibody? its preety urgent. I am awaiting to hear from you in your best conveniuence!!

Asked by: Mahmud
Thank you for your question. We do not have a negative control peptide for this antibody, but we would suggest using a normal mouse IgG2a (https://www.scbt.com/p/normal-mouse-igg2a) as a negative control for this LOX antibody. You can find the appropriate normal mouse IgG for your LMO2 antibody here: https://www.scbt.com/browse/Control-IgGs-and-IgG-Conjugates/_/N-1j3ajah
Answered by: Tech Service
Date published: 2020-02-13

Can the LOX (F-8): sc-373995 mouse monoclonal antibody be used for immunohistochemical staining of mouse tissue?

Asked by: cjMara
Yes. For the best results, we recommend using either a directly conjugated monoclonal antibody, or our highly specific mouse IgG binding protein conjugated to HRP (sc-516102). This primary antibody is available directly conjugated to HRP, PE, FITC, Alexa Fluor® 488, or Alexa Fluor® 647.
Answered by: Technical Support 15
Date published: 2017-01-22
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Rated 4 out of 5 by from Work well in WB analysis This antibody works well in western blot analysis of mouse liver lysate
Date published: 2017-10-02
Rated 4 out of 5 by from Good antibody I used this antibody in liver cell line. The major band located in 50KD.
Date published: 2017-08-21
Rated 5 out of 5 by from both extracellular and nuclear lox can be detected In our hands, this antibody detect extra cellular Lox well and nuclear Lox either.
Date published: 2017-02-13
Rated 5 out of 5 by from Outstanding Great product for western blot, IF and IHC staining applications
Date published: 2017-02-05
Rated 5 out of 5 by from Wise choice in antibody... This LOX antibody will not disappoint! Strong for WB and IF, but also specific and reliable for IHC.
Date published: 2017-01-09
Rated 5 out of 5 by from Great bands seen in WB using WI Great bands seen in WB using WI-38 and MCF7 whole cell lysates. -SCBT QC
Date published: 2015-05-16
Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic and nuclear Superb immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human skin tissue. -SCBT QC
Date published: 2015-05-13
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