Laminin beta-1 Antibody (A-1) recommended for detection of Laminin β-1 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Laminin β-1 Antibody (A-1): sc-17810

Laminin β-1 Antibody (A-1) is rated 4.3 out of 5 by 8.
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Datasheets

    • Anti-Laminin β-1 Antibody (A-1) is a mouse monoclonal IgG3 (kappa light chain) Laminin β-1 antibody provided at 200 µg/ml
    • raised against amino acids 1487-1786 mapping at the C-terminus of Laminin β-1 of human origin
    • recommended for detection of Laminin β-1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Laminin β-1 (A-1): sc-17810.
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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What is the recommended fixation method for immunofluorescence staining with Laminin β-1 (A-1): sc-17810 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Did not work for western blot Used a 1:300 dilution for WB with Sum159, Caco-2, and 293T cell lysates. No bands were observed.
Date published: 2017-11-30
Rated 4 out of 5 by from Very good for IHC(Fr) Staining for PFA-fixed and frozen mouse bone marrow
Date published: 2017-07-19
Rated 4 out of 5 by from Works well on blots; see image We compared the A-1 mouse monoclonal antibody to laminin beta1 with the older LT3 rat monoclonal antibody using secondary antibodies to mouse and rat respectively. The tissue was a homogenate of mouse choroid plexus, a rich source of laminin beta1, that had been frozen and thawed. Both antibodies worked well, but both anti-rat IgG and the mouse kappa BP stained lower MW bands. They may be fragments of laminin beta1, as they seem to migrate above the molecular weights of heavy and light chains, but more testing is needed. We have not yet tested it for immunofluorescence.
Date published: 2016-12-15
Rated 5 out of 5 by from Produced superb immunofluorescence basement Produced superb immunofluorescence basement membrane and trabecular meshwork staining in normal mouse eye frozen section. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Robust signal in WB in NIH/3T3 whole cell lysates Robust signal in WB in NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-03-15
Rated 5 out of 5 by from Strong WB band in rat PC12 cells Strong WB band in rat PC12 cells. -SCBT Publication Review
Date published: 2015-02-10
Rated 5 out of 5 by from Good publishable data in WB in mouse ES cells Good publishable data in WB in mouse ES cells. -SCBT Publication Review
Date published: 2015-01-22
Rated 5 out of 5 by from Good CoIP antibody in human PC Good CoIP antibody in human PC-3 cells. -SCBT Publication Review
Date published: 2015-01-02
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