Laminin β-1 Antibody (LT3): sc-33709

Laminin β-1 Antibody (LT3) is a rat monoclonal IgG1 antibody that detects Laminin β-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Laminin β-1 antibody (LT3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Laminin β-1 plays a crucial role in the formation and maintenance of basement membranes, which are specialized extracellular matrices that provide structural support to tissues and influence cellular behaviors such as proliferation, migration, and differentiation. These basement membranes are composed of laminins, which are heterotrimeric proteins made up of α, β, and γ chains, and they interact with various cell surface receptors, including integrins and dystroglycan, to mediate cell adhesion and signaling. The proper assembly and function of basement membranes are vital for tissue integrity and repair, as they serve as a barrier and scaffold for cells. The human Laminin β-1 gene is located on chromosome 7q22 and is expressed in a wide range of tissues, highlighting its importance in both normal physiology and pathological conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Laminin β-1 Antibody (LT3) References:

1. Role of laminin isoforms in glomerular structure.  |  Hansen, K. and Abrass, CK. 1999. Pathobiology. 67: 84-91. PMID: 10023136
2. Form and function: the laminin family of heterotrimers.  |  Colognato, H. and Yurchenco, PD. 2000. Dev Dyn. 218: 213-34. PMID: 10842354
3. Transcriptional regulation of laminin gene expression.  |  Aberdam, D., et al. 2000. Microsc Res Tech. 51: 228-37. PMID: 11054873
4. Laminin-β1 impairs spatial learning through inhibition of ERK/MAPK and SGK1 signaling.  |  Yang, YC., et al. 2011. Neuropsychopharmacology. 36: 2571-86. PMID: 21849984
5. Crystal structures of the network-forming short-arm tips of the laminin β1 and γ1 chains.  |  Carafoli, F., et al. 2012. PLoS One. 7: e42473. PMID: 22860131
6. Phosphorylation mapping of Laminin β1-chain: Kinases in association with active sites.  |  Verrou, KM., et al. 2019. J Biosci. 44: PMID: 31180068
7. CRISPR-Cas9-mediated labelling of the C-terminus of human laminin β1 leads to secretion inhibition.  |  Shaw, L., et al. 2020. BMC Res Notes. 13: 90. PMID: 32085798
8. The laminin family.  |  Tryggvason, K. 1993. Curr Opin Cell Biol. 5: 877-82. PMID: 8240830
9. Role of laminin in endothelial cell recognition and differentiation.  |  Schnaper, HW., et al. 1993. Kidney Int. 43: 20-5. PMID: 8433560
10. Domains of laminin.  |  Engvall, E. and Wewer, UM. 1996. J Cell Biochem. 61: 493-501. PMID: 8806072
11. Laminin and the mechanism of neuronal outgrowth.  |  Luckenbill-Edds, L. 1997. Brain Res Brain Res Rev. 23: 1-27. PMID: 9063584
12. Laminin isoforms and epithelial development.  |  Ekblom, M., et al. 1998. Ann N Y Acad Sci. 857: 194-211. PMID: 9917842

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Laminin β-1 Antibody (LT3) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-Laminin β-1 Antibody (LT3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Laminin β-1 Antibody (LT3) Specifications

Alternate Product Name: Laminin β-1

Product Clone Name: LT3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: Laminin β-1

Epitope Species: mouse

Product Reactivity/Detection: Laminin β-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for Laminin β-1 Antibody (LT3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin