Laminin beta-1 Antibody (LT3) recommended for detection of Laminin β-1 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Laminin β-1 Antibody (LT3): sc-33709

Laminin β-1 Antibody (LT3) is rated 4.0 out of 5 by 6.
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Datasheets

    • Laminin β-1 Antibody (LT3) is a rat monoclonal IgG1 provided at 200 µg/ml
    • raised against partially purified preparation of laminin from the EHS mouse tumor
    • Anti-Laminin beta-1 Antibody (LT3) is recommended for detection of Laminin β-1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-Laminin beta-1 Antibody (LT3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Laminin β-1 (LT3): sc-33709.
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Dear sir/madam, I would like to get a free sample of the Laminin β-1 Antibody (LT3): sc-33709. Thanks in advance, Christa Tanganyika

Asked by: christa
Thank you for your question. If you have an interest in testing a free 10 µg sample of sc-33709, please contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

Which product is recommended for kidney regeneration?

Asked by: Rupert13
Thank you for your question. Unfortunately we are not able to provide that information. Our antibodies are for research use only. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-04-14

What is the recommended fixation method for immunofluorescence staining with Laminin β-1 (LT3): sc-33709 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from IF do not work on frozen sections Tested IF on frozen sections of 4% PFA fixed mouse cerebellum, no specific signal was detected.
Date published: 2018-07-30
Rated 4 out of 5 by from Great Western blot data of Laminin We used this antibody to detect laminin beta 1 and got a good result. Fresh liver samples were homogenized in RIPA buffer, then 40ug of total protein were subjected to 10% gel for SDS-PAGE and transferred onto a PVDF membrane. The membrane were incubated overnight at 4℃ with laminin beta 1 antibodies(1:1000) and it were incubated with anti- rat IgG(1:2000) at room temperature and detected with ECL kit.
Date published: 2017-06-07
Rated 5 out of 5 by from Works well This antibody performs well in staining and blotting procedures. Signal is clear and specific and will help you achieve your desired outcome.
Date published: 2017-02-07
Rated 4 out of 5 by from Seems to work well in our Western but we Seems to work well in our Western but we have not fully tested.
Date published: 2015-07-28
Rated 5 out of 5 by from Produced superb immunofluorescence basement Produced superb immunofluorescence basement membrane, Descemet s membrane and smooth muscle staining in normal mouse eye frozen section. -SCBT QC
Date published: 2015-05-10
Rated 5 out of 5 by from Strong signal for WB in T98G whole cell lysate Strong signal for WB in T98G whole cell lysate. -SCBT QC
Date published: 2015-04-27
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