Laminin alpha-2 Antibody (B-4) recommended for detection of Laminin α-2 of mouse, rat and human origin by WB, IP, IF and ELISA

Laminin α-2 Antibody (B-4): sc-55605

Laminin α-2 Antibody (B-4) is rated 5.0 out of 5 by 2.
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Datasheets

    • Anti-Laminin α-2 Antibody (B-4) is a mouse monoclonal IgG1 (kappa light chain) Laminin α-2 antibody provided at 200 µg/ml
    • raised against amino acids 2811-2940 mapping near the C-terminus of Laminin α-2 of human origin
    • Anti-Laminin alpha-2 Antibody (B-4) is recommended for detection of Laminin α-2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Laminin alpha-2 Antibody (B-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Laminin α-2 (B-4): sc-55605.
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I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Laminin α-2, sc-55605?

Asked by: TinTin
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service
Date published: 2017-01-27

Hello, I have a question about Laminin α-2 Antibody (B-4): sc-55605; I want to know if this antibody can used in 2 western blot and Immunohistochemistry techniques?

Asked by: Mahdieh
Thank you for your question. Laminin α-2 Antibody (B-4) is a mouse monoclonal that is recommended for detection of Laminin α-2 of mouse, rat and human origin by WB (Western blot) and IF (immunofluorescence or immunohistochemistry on fresh or frozen tissues or cells fixed in methanol or acetone). This antibody has not yet been tested on formalin-fixed paraffin-embedded tissue sections.
Answered by: Technical Support
Date published: 2016-12-14
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Rated 5 out of 5 by from Clear strong bands in WB Very clean and distinct strong bands in Caki-1 and HeLa whole cell lysates and rat lung tissue extract.
Date published: 2016-12-23
Rated 5 out of 5 by from Great Western blot data of Laminin α Great Western blot data of Laminin α-2 expression in mouse embryo tissue extract. -SCBT QC
Date published: 2013-06-30
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