Laminin α-2 Antibody (4H8-2): sc-59854

Laminin α-2 Antibody (4H8-2) is a rat monoclonal IgG1 antibody that detects Laminin α-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Laminin α-2 antibody (4H8-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Laminins, including Laminin α-2, are crucial structural components of basement membranes, which are specialized extracellular matrices that provide support and anchorage to cells. These basement membranes are not only found beneath epithelial layers but also envelop blood vessels, muscle, peripheral nerves, and adipose tissue, playing a vital role in maintaining tissue integrity and function. The unique structure of Laminin α-2, which consists of a heterotrimeric assembly of α, β, and γ chains, allows interaction with various cell surface receptors, including integrins and dystroglycan, facilitating cell adhesion and signaling. Mutations in the Laminin α-2 gene are linked to congenital muscular dystrophy in humans and dystrophia muscularis in mice, highlighting its significance in muscle development and pathology.

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Laminin α-2 Antibody (4H8-2) References:

1. Role of laminin isoforms in glomerular structure.  |  Hansen, K. and Abrass, CK. 1999. Pathobiology. 67: 84-91. PMID: 10023136
2. Clinical correlations in 16 patients with total or partial laminin alpha2 deficiency characterized using antibodies against 2 fragments of the protein.  |  Morandi, L., et al. 1999. Arch Neurol. 56: 209-15. PMID: 10025426
3. Form and function: the laminin family of heterotrimers.  |  Colognato, H. and Yurchenco, PD. 2000. Dev Dyn. 218: 213-34. PMID: 10842354
4. Transcriptional regulation of laminin gene expression.  |  Aberdam, D., et al. 2000. Microsc Res Tech. 51: 228-37. PMID: 11054873
5. Laminin alpha 2 (merosin)-deficient muscular dystrophy and demyelinating neuropathy in two cats.  |  O'Brien, DP., et al. 2001. J Neurol Sci. 189: 37-43. PMID: 11535231
6. Laminin-α2 Chain-Deficient Congenital Muscular Dystrophy: Pathophysiology and Development of Treatment.  |  Durbeej, M. 2015. Curr Top Membr. 76: 31-60. PMID: 26610911
7. Laminin Alpha 2 Enhances the Protective Effect of Exosomes on Human iPsc-Derived Cardiomyocytes in an In Vitro Ischemia-Reoxygenation Model.  |  Mesquita, FCP., et al. 2024. Int J Mol Sci. 25: PMID: 38612582
8. The laminin family.  |  Tryggvason, K. 1993. Curr Opin Cell Biol. 5: 877-82. PMID: 8240830
9. Role of laminin in endothelial cell recognition and differentiation.  |  Schnaper, HW., et al. 1993. Kidney Int. 43: 20-5. PMID: 8433560
10. Domains of laminin.  |  Engvall, E. and Wewer, UM. 1996. J Cell Biochem. 61: 493-501. PMID: 8806072
11. Disruption of the lama2 gene in embryonic stem cells: laminin alpha 2 is necessary for sustenance of mature muscle cells.  |  Kuang, W., et al. 1998. Exp Cell Res. 241: 117-25. PMID: 9633519
12. Laminin isoforms and epithelial development.  |  Ekblom, M., et al. 1998. Ann N Y Acad Sci. 857: 194-211. PMID: 9917842

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Laminin α-2 Antibody (4H8-2) has 59 citations in a variety of scientific publications.

Primary antibodies like Anti-Laminin α-2 Antibody (4H8-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Laminin α-2 Antibody (4H8-2) Specifications

Alternate Product Name: Laminin α-2

Product Clone Name: 4H8-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: Laminin α-2

Epitope Species: mouse

Product Reactivity/Detection: Laminin α-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 59 citations in various scientific publications

Available Conjugates/Forms for Laminin α-2 Antibody (4H8-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Laminin α-2 antibody is Laminin α-2 Antibody (B-4): sc-55605.