LAGE-3 Antibody (E-2): sc-515776
- LAGE-3 Antibody (E-2) is a mouse monoclonal IgG2b κ LAGE-3 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1-20 at the N-terminus of LAGE-3 of mouse origin
- LAGE-3 Antibody (E-2) is recommended for detection of LAGE-3 of mouse and rat origin by WB, IP, IF and ELISA
- Anti-LAGE-3 Antibody (E-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for LAGE-3 Antibody (E-2) for WB applications. These reagents are now offered in bundles with LAGE-3 Antibody (E-2) (see ordering information below).
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LAGE-3 Antibody (E-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects LAGE-3 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LAGE-3 (E-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LAGE-3, also known as L antigen family member 3, DXS9879E, ESO3, or ITBA2, is a 143 amino acid protein that belongs to the cancer/testis antigen (CTAG) family. Notably, LAGE-3 is expressed in a wide range of somatic tissues, unlike other family members that are typically restricted to testicular expression and activated in various human tumors. This ubiquitous expression suggests that LAGE-3 may play a critical role in normal cellular functions, potentially influencing processes such as cell proliferation and differentiation. The gene encoding LAGE-3 is located on mouse chromosome X A7.3, and its high conservation across species indicates its functional importance. Additionally, an intronless pseudogene with significant sequence similarity to LAGE-3 is found on human chromosome 9, highlighting the evolutionary significance of this protein.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
LAGE-3 Antibody (E-2) References:
- Interleukin-2-induced, melanoma-specific T cells recognize CAMEL, an unexpected translation product of LAGE-1. | Aarnoudse, CA., et al. 1999. Int J Cancer. 82: 442-8. PMID: 10399963
- Multiple pathogenic and benign genomic rearrangements occur at a 35 kb duplication involving the NEMO and LAGE2 genes. | Aradhya, S., et al. 2001. Hum Mol Genet. 10: 2557-67. PMID: 11709543
- A new member of the NY-ESO-1 gene family is ubiquitously expressed in somatic tissues and evolutionarily conserved. | Alpen, B., et al. 2002. Gene. 297: 141-9. PMID: 12384295
- Refinement of the X-linked nonsyndromic high-grade myopia locus MYP1 on Xq28 and exclusion of 13 known positional candidate genes by direct sequencing. | Ratnamala, U., et al. 2011. Invest Ophthalmol Vis Sci. 52: 6814-9. PMID: 21357393
- Characterization and fine localization of two new genes in Xq28 using the genomic sequence/EST database screening approach. | Faranda, S., et al. 1996. Genomics. 34: 323-7. PMID: 8786131
- Genomic cloning and localization of CTAG, a gene encoding an autoimmunogenic cancer-testis antigen NY-ESO-1, to human chromosome Xq28. | Chen, YT., et al. 1997. Cytogenet Cell Genet. 79: 237-40. PMID: 9605863
- LAGE-1, a new gene with tumor specificity. | Lethé, B., et al. 1998. Int J Cancer. 76: 903-8. PMID: 9626360
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LAGE-3 Antibody (E-2) | sc-515776 | 200 µg/ml | $322.00 | |||
LAGE-3 Antibody (E-2): m-IgG Fc BP-HRP Bundle | sc-531509 | 200 µg Ab; 10 µg BP | $361.00 | |||
LAGE-3 Antibody (E-2): m-IgGκ BP-HRP Bundle | sc-525209 | 200 µg Ab, 40 µg BP | $361.00 | |||
LAGE-3 Antibody (E-2): m-IgG2b BP-HRP Bundle | sc-550292 | 200 µg Ab; 10 µg BP | $361.00 | |||
LAGE-3 Antibody (E-2) AC | sc-515776 AC | 500 µg/ml, 25% agarose | $424.00 | |||
LAGE-3 Antibody (E-2) HRP | sc-515776 HRP | 200 µg/ml | $322.00 | |||
LAGE-3 Antibody (E-2) FITC | sc-515776 FITC | 200 µg/ml | $336.00 | |||
LAGE-3 Antibody (E-2) PE | sc-515776 PE | 200 µg/ml | $349.00 | |||
LAGE-3 Antibody (E-2) Alexa Fluor® 488 | sc-515776 AF488 | 200 µg/ml | $364.00 | |||
LAGE-3 Antibody (E-2) Alexa Fluor® 546 | sc-515776 AF546 | 200 µg/ml | $364.00 | |||
LAGE-3 Antibody (E-2) Alexa Fluor® 594 | sc-515776 AF594 | 200 µg/ml | $364.00 | |||
LAGE-3 Antibody (E-2) Alexa Fluor® 647 | sc-515776 AF647 | 200 µg/ml | $364.00 | |||
LAGE-3 Antibody (E-2) Alexa Fluor® 680 | sc-515776 AF680 | 200 µg/ml | $364.00 | |||
LAGE-3 Antibody (E-2) Alexa Fluor® 790 | sc-515776 AF790 | 200 µg/ml | $364.00 |