L-Caldesmon Antibody (F-1) recommended for detection of L-caldesmon of human origin by WB, IP, IF and ELISA

L-Caldesmon Antibody (F-1): sc-48427

L-Caldesmon Antibody (F-1) is rated 5.0 out of 5 by 3.
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Datasheets

    • L-Caldesmon Antibody (F-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 494-793 of L-caldesmon of human origin
    • recommended for detection of L-caldesmon of human origin by WB, IP, IF and ELISA

What is the recommended fixation method for immunofluorescence staining with L-Caldesmon (F-1): sc-48427 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Works well for western blotting with transfected Works well for western blotting with transfected lysates. -SCBT QC
Date published: 2015-06-02
Rated 5 out of 5 by from Produced exceptional immunofluorescence membrane Produced exceptional immunofluorescence membrane and cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-05-19
Rated 5 out of 5 by from Clean bands in western blot analysis of L Clean bands in western blot analysis of L-Caldesmon expression in HeLa and JAR whole cell lysates. -SCBT QC
Date published: 2015-01-04
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