Anti-H-Caldesmon Antibody (h-CALD) recommended for detection of H-Caldesmon of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-H-Caldesmon Antibody (h-CALD): sc-58703

Anti-H-Caldesmon Antibody (h-CALD) is rated 4.7 out of 5 by 3.
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Datasheets

    • Anti-H-Caldesmon Antibody (h-CALD) is a mouse monoclonal IgG1 κ H-Caldesmon antibody, cited in 5 publications, provided at 200 µg/ml
    • raised against crude uterus extract of human origin
    • Anti-H-Caldesmon Antibody (h-CALD) is recommended for detection of H-Caldesmon of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-H-Caldesmon Antibody (h-CALD) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of H-Caldesmon (h-CALD): sc-58703.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) are the preferred secondary detection reagents for H-Caldesmon Antibody (h-CALD) for WB and IHC(P) applications. These reagents are now offered in bundles with H-Caldesmon Antibody (h-CALD) (see ordering information below).
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I am using H-Caldesmon (h-CALD): sc-58703 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Great antibody I used this antibody for IHC. the result was great.
Date published: 2020-08-17
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic staining Excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human ovary tissue. -SCBT QC
Date published: 2015-05-10
Rated 5 out of 5 by from Clean Clean, clear bands in western blot analysis of H-Caldesmon expression in HISM and FCH whole cell lysates. -SCBT QC
Date published: 2015-03-04
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