Ku70 Antibody (A-9): sc-5309

Ku70 Antibody (A-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects Ku70 protein of human origin through various applications, including western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, flow cytometry (FCM), and enzyme-linked immunosorbent assay. Ku70 (A-9) antibody is offered in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and various Alexa Fluor® conjugates. Ku70 protein, located in the nucleus, plays a crucial role in DNA repair and maintenance of genomic stability. Ku70 forms a heterodimeric complex with Ku86, which is essential for the recognition and binding of DNA double-strand breaks, thereby facilitating the non-homologous end joining (NHEJ) repair pathway. This function is vital for cellular response to DNA damage, as Ku70 helps prevent mutations and maintains the integrity of the genome, which is particularly important in the context of cancer and other genetic disorders. Ku70 protein also participates in transcriptional regulation, influencing the expression of genes involved in cell cycle control and apoptosis. Using Ku70 (A-9) monoclonal antibody in research can provide insights into the mechanisms of DNA repair and the implications of Ku70 in various diseases, making Ku70 (A-9) antibody an invaluable tool for studying cellular responses to DNA damage.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Ku70 Antibody (A-9) References:

1. Cell surface expression of the 70-kD component of Ku, a DNA-binding nuclear autoantigen.  |  Prabhakar, BS., et al. 1990. J Clin Invest. 86: 1301-5. PMID: 2212014
2. The autoantigen Ku is indistinguishable from NF IV, a protein forming multimeric protein-DNA complexes.  |  Stuiver, MH., et al. 1990. J Exp Med. 172: 1049-54. PMID: 2212941
3. Molecular cloning of cDNA encoding the p70 (Ku) lupus autoantigen.  |  Reeves, WH. and Sthoeger, ZM. 1989. J Biol Chem. 264: 5047-52. PMID: 2466842
4. cDNA-derived amino acid sequence of the 86-kDa subunit of the Ku antigen.  |  Yaneva, M., et al. 1989. J Biol Chem. 264: 13407-11. PMID: 2760028
5. Cloning and characterization of a cDNA that encodes a 70-kDa novel human thyroid autoantigen.  |  Chan, JY., et al. 1989. J Biol Chem. 264: 3651-4. PMID: 2917966
6. CIRKIL Exacerbates Cardiac Ischemia/Reperfusion Injury by Interacting With Ku70.  |  Xiao, H., et al. 2022. Circ Res. 130: e3-e17. PMID: 35105170
7. Characterization of the DNA-binding protein antigen Ku recognized by autoantibodies from patients with rheumatic disorders.  |  Mimori, T., et al. 1986. J Biol Chem. 261: 2274-8. PMID: 3511059
8. SETD4-mediated KU70 methylation suppresses apoptosis.  |  Wang, Y., et al. 2022. Cell Rep. 39: 110794. PMID: 35545041
9. Ku70 Binding to YAP Alters PARP1 Ubiquitination to Regulate Genome Stability and Tumorigenesis.  |  Shu, Y., et al. 2024. Cancer Res. 84: 2836-2855. PMID: 38862269
10. Characterization of a high molecular weight acidic nuclear protein recognized by autoantibodies in sera from patients with polymyositis-scleroderma overlap.  |  Mimori, T., et al. 1981. J Clin Invest. 68: 611-20. PMID: 7276162
11. Enhancer 1 binding factor, a Ku-related protein, is a positive regulator of RNA polymerase I transcription initiation.  |  Hoff, CM., et al. 1994. Proc Natl Acad Sci U S A. 91: 762-6. PMID: 8290597
12. The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen.  |  Gottlieb, TM. and Jackson, SP. 1993. Cell. 72: 131-42. PMID: 8422676

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ku70 Antibody (A-9) has 78 citations in a variety of scientific publications.

Primary antibodies like Anti-Ku70 Antibody (A-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ku70 Antibody (A-9) Specifications

Alternate Product Name: KU70

Product Clone Name: A-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 302-609

Epitope Target: Ku70

Epitope Species: human

Product Reactivity/Detection: Ku70

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 78 citations in various scientific publications

Available Conjugates/Forms for Ku70 Antibody (A-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin