Klotho Antibody (F-5): sc-515939
- Klotho Antibody (F-5) is a mouse monoclonal IgG2b κ, cited in 4 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 119-143 within an internal region of Klotho of human origin
- Klotho Antibody (F-5) is recommended for detection of Klotho of human origin by WB, IP, IF and ELISA
- Anti-Klotho Antibody (F-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Klotho (F-5): sc-515939.
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Klotho Antibody (F-5) for WB applications. These reagents are now offered in bundles with Klotho Antibody (F-5) (see ordering information below).
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Klotho Antibody (F-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects Klotho of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Klotho antibody (F-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Klotho is a type I transmembrane protein that plays a crucial role in regulating aging and longevity, with expression being particularly abundant in the kidney and brain. Klotho is involved in several important physiological processes, including the regulation of mineral metabolism and the modulation of insulin signaling. Notably, Klotho acts as a co-receptor for fibroblast growth factors, which are essential for various cellular functions, including cell growth, differentiation, and survival. The interaction of Klotho with these growth factors highlights Klotho′s significance in maintaining cellular homeostasis and potential implications in age-related diseases. The human Klotho gene, located on chromosome 13q12, encodes a 1012 amino acid protein that exhibits approximately 80% homology with its mouse counterpart. Deficiencies in Klotho expression have been linked to various systemic phenotypes that resemble human aging, such as arteriosclerosis and osteoporosis, underscoring Klotho′s importance in both health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Klotho Antibody (F-5) References:
- Disruption of klotho gene causes an abnormal energy homeostasis in mice. | Mori, K., et al. 2000. Biochem Biophys Res Commun. 278: 665-70. PMID: 11095966
- Severely reduced production of klotho in human chronic renal failure kidney. | Koh, N., et al. 2001. Biochem Biophys Res Commun. 280: 1015-20. PMID: 11162628
- Upregulation of the klotho gene expression by thyroid hormone and during adipose differentiation in 3T3-L1 adipocytes. | Mizuno, I., et al. 2001. Life Sci. 68: 2917-23. PMID: 11411791
- Regulation of angiogenesis by the aging suppressor gene klotho. | Fukino, K., et al. 2002. Biochem Biophys Res Commun. 293: 332-7. PMID: 12054604
- Molecular basis of Klotho: from gene to function in aging. | Xu, Y. and Sun, Z. 2015. Endocr Rev. 36: 174-93. PMID: 25695404
- Klotho in Clinical Nephrology: Diagnostic and Therapeutic Implications. | Neyra, JA., et al. 2020. Clin J Am Soc Nephrol. 16: 162-176. PMID: 32699047
- Klotho, Aging, and the Failing Kidney. | Buchanan, S., et al. 2020. Front Endocrinol (Lausanne). 11: 560. PMID: 32982966
- Klotho Deficiency Causes Heart Aging via Impairing the Nrf2-GR Pathway. | Chen, K., et al. 2021. Circ Res. 128: 492-507. PMID: 33334122
- Pathobiology of the Klotho Antiaging Protein and Therapeutic Considerations. | Prud'homme, GJ., et al. 2022. Front Aging. 3: 931331. PMID: 35903083
- Klotho, Oxidative Stress, and Mitochondrial Damage in Kidney Disease. | Donate-Correa, J., et al. 2023. Antioxidants (Basel). 12: PMID: 36829798
- Longevity factor klotho enhances cognition in aged nonhuman primates. | Castner, SA., et al. 2023. Nat Aging. 3: 931-937. PMID: 37400721
- FGF23 and klotho at the intersection of kidney and cardiovascular disease. | Edmonston, D., et al. 2024. Nat Rev Cardiol. 21: 11-24. PMID: 37443358
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Klotho Antibody (F-5) | sc-515939 | 200 µg/ml | $316.00 | |||
Klotho Antibody (F-5): m-IgGκ BP-HRP Bundle | sc-525291 | 200 µg Ab, 40 µg BP | $354.00 | |||
Klotho Antibody (F-5): m-IgG2b BP-HRP Bundle | sc-549226 | 200 µg Ab; 10 µg BP | $354.00 | |||
Klotho Antibody (F-5) AC | sc-515939 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Klotho Antibody (F-5) HRP | sc-515939 HRP | 200 µg/ml | $316.00 | |||
Klotho Antibody (F-5) FITC | sc-515939 FITC | 200 µg/ml | $330.00 | |||
Klotho Antibody (F-5) PE | sc-515939 PE | 200 µg/ml | $343.00 | |||
Klotho Antibody (F-5) Alexa Fluor® 488 | sc-515939 AF488 | 200 µg/ml | $357.00 | |||
Klotho Antibody (F-5) Alexa Fluor® 546 | sc-515939 AF546 | 200 µg/ml | $357.00 | |||
Klotho Antibody (F-5) Alexa Fluor® 594 | sc-515939 AF594 | 200 µg/ml | $357.00 | |||
Klotho Antibody (F-5) Alexa Fluor® 647 | sc-515939 AF647 | 200 µg/ml | $357.00 | |||
Klotho Antibody (F-5) Alexa Fluor® 680 | sc-515939 AF680 | 200 µg/ml | $357.00 | |||
Klotho Antibody (F-5) Alexa Fluor® 790 | sc-515939 AF790 | 200 µg/ml | $357.00 |