KLHL31 Antibody (E-2): sc-514464
- KLHL31 Antibody (E-2) is a mouse monoclonal IgG2a κ KLHL31 antibody provided at 200 µg/ml
- raised against amino acids 261-365 mapping within an internal region of KLHL31 of human origin
- KLHL31 Antibody (E-2) is recommended for detection of KLHL31 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-KLHL31 Antibody (E-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for KLHL31 Antibody (E-2) for WB applications. These reagents are now offered in bundles with KLHL31 Antibody (E-2) (see ordering information below).
KLHL31 Antibody (E-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects KLHL31 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). KLHL31 Antibody (E-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. KLHL31, also known as kelch-like protein 31, is a 634 amino acid protein that plays a crucial role in cellular processes by functioning as an E3 ubiquitin ligase, which is essential for protein degradation and regulation of various signaling pathways. KLHL31 is predominantly expressed in skeletal muscle and exhibits weaker expression in the heart, highlighting its importance in muscle development and function. KLHL31 structure includes one BACK (BTB/kelch associated) domain and six kelch repeats, which are critical for substrate protein interaction and E3 ligase complex formation. The BTB domain suggests KLHL31 may also influence chromatin structure and function for transcriptional regulation, thereby impacting gene expression. anti-KLHL31 antibody (E-2) serves as an invaluable tool for researchers studying muscle biology and related signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
KLHL31 Antibody (E-2) References:
- The kelch repeat superfamily of proteins: propellers of cell function. | Adams, J., et al. 2000. Trends Cell Biol. 10: 17-24. PMID: 10603472
- In-depth mutational analysis of the promyelocytic leukemia zinc finger BTB/POZ domain reveals motifs and residues required for biological and transcriptional functions. | Melnick, A., et al. 2000. Mol Cell Biol. 20: 6550-67. PMID: 10938130
- Molecular phylogeny of the kelch-repeat superfamily reveals an expansion of BTB/kelch proteins in animals. | Prag, S. and Adams, JC. 2003. BMC Bioinformatics. 4: 42. PMID: 13678422
- BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases. | Geyer, R., et al. 2003. Mol Cell. 12: 783-90. PMID: 14527422
- A novel zebrafish kelchlike gene klhl and its human ortholog KLHL display conserved expression patterns in skeletal and cardiac muscles. | Wu, YL. and Gong, Z. 2004. Gene. 338: 75-83. PMID: 15302408
- The BACK domain in BTB-kelch proteins. | Stogios, PJ. and Privé, GG. 2004. Trends Biochem Sci. 29: 634-7. PMID: 15544948
- The BTB/POZ domain: a new protein-protein interaction motif common to DNA- and actin-binding proteins. | Albagli, O., et al. 1995. Cell Growth Differ. 6: 1193-8. PMID: 8519696
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KLHL31 Antibody (E-2) | sc-514464 | 200 µg/ml | $322.00 | |||
KLHL31 Antibody (E-2): m-IgG Fc BP-HRP Bundle | sc-531138 | 200 µg Ab; 10 µg BP | $361.00 | |||
KLHL31 Antibody (E-2): m-IgGκ BP-HRP Bundle | sc-524738 | 200 µg Ab, 40 µg BP | $361.00 | |||
KLHL31 Antibody (E-2): m-IgG2a BP-HRP Bundle | sc-547699 | 200 µg Ab; 10 µg BP | $361.00 | |||
KLHL31 Antibody (E-2) AC | sc-514464 AC | 500 µg/ml, 25% agarose | $424.00 | |||
KLHL31 Antibody (E-2) HRP | sc-514464 HRP | 200 µg/ml | $322.00 | |||
KLHL31 Antibody (E-2) FITC | sc-514464 FITC | 200 µg/ml | $336.00 | |||
KLHL31 Antibody (E-2) PE | sc-514464 PE | 200 µg/ml | $349.00 | |||
KLHL31 Antibody (E-2) Alexa Fluor® 488 | sc-514464 AF488 | 200 µg/ml | $364.00 | |||
KLHL31 Antibody (E-2) Alexa Fluor® 546 | sc-514464 AF546 | 200 µg/ml | $364.00 | |||
KLHL31 Antibody (E-2) Alexa Fluor® 594 | sc-514464 AF594 | 200 µg/ml | $364.00 | |||
KLHL31 Antibody (E-2) Alexa Fluor® 647 | sc-514464 AF647 | 200 µg/ml | $364.00 | |||
KLHL31 Antibody (E-2) Alexa Fluor® 680 | sc-514464 AF680 | 200 µg/ml | $364.00 | |||
KLHL31 Antibody (E-2) Alexa Fluor® 790 | sc-514464 AF790 | 200 µg/ml | $364.00 |