KLHL20 Antibody (D-7): sc-515381
- KLHL20 Antibody (D-7) is a mouse monoclonal IgG1 κ KLHL20 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 468-557 mapping within an internal region of KLHL20 of human origin
- KLHL20 Antibody (D-7) is recommended for detection of KLHL20 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-KLHL20 Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for KLHL20 Antibody (D-7) for WB applications. These reagents are now offered in bundles with KLHL20 Antibody (D-7) (see ordering information below).
KLHL20 Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects KLHL20 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). KLHL20 (D-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. KLHL20 protein, also known as kelch-like 20, KHLHX, KLEIP, or KLHLX, consists of 609 amino acids and is structurally characterized by the presence of six kelch repeats, one BACK (BTB/Kelch associated) domain, and one BTB (POZ) domain. The BTB domain plays a crucial role in protein homodimerization and is implicated in transcriptional regulation through chromatin structure and function modulation. KLHL20 acts as a substrate-specific adapter for an E3 ubiquitin-protein ligase complex, facilitating ubiquitination and subsequent proteasomal degradation of target proteins, thereby influencing various cellular processes. KLHL20′s ability to regulate protein levels through ubiquitination underscores its significance in maintaining cellular homeostasis and potential involvement in pathological conditions when dysregulated.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
KLHL20 Antibody (D-7) References:
- The kelch repeat superfamily of proteins: propellers of cell function. | Adams, J., et al. 2000. Trends Cell Biol. 10: 17-24. PMID: 10603472
- In-depth mutational analysis of the promyelocytic leukemia zinc finger BTB/POZ domain reveals motifs and residues required for biological and transcriptional functions. | Melnick, A., et al. 2000. Mol Cell Biol. 20: 6550-67. PMID: 10938130
- Drosophila Kelch regulates actin organization via Src64-dependent tyrosine phosphorylation. | Kelso, RJ., et al. 2002. J Cell Biol. 156: 703-13. PMID: 11854310
- Molecular phylogeny of the kelch-repeat superfamily reveals an expansion of BTB/kelch proteins in animals. | Prag, S. and Adams, JC. 2003. BMC Bioinformatics. 4: 42. PMID: 13678422
- BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases. | Geyer, R., et al. 2003. Mol Cell. 12: 783-90. PMID: 14527422
- Domains of Importin-alpha2 required for ring canal assembly during Drosophila oogenesis. | Gorjánácz, M., et al. 2006. J Struct Biol. 154: 27-41. PMID: 16458020
- The BTB/POZ domain: a new protein-protein interaction motif common to DNA- and actin-binding proteins. | Albagli, O., et al. 1995. Cell Growth Differ. 6: 1193-8. PMID: 8519696
- Drosophila kelch is an oligomeric ring canal actin organizer. | Robinson, DN. and Cooley, L. 1997. J Cell Biol. 138: 799-810. PMID: 9265647
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KLHL20 Antibody (D-7) | sc-515381 | 200 µg/ml | $322.00 | |||
KLHL20 Antibody (D-7): m-IgG Fc BP-HRP Bundle | sc-531349 | 200 µg Ab; 10 µg BP | $361.00 | |||
KLHL20 Antibody (D-7): m-IgGκ BP-HRP Bundle | sc-525017 | 200 µg Ab, 40 µg BP | $361.00 | |||
KLHL20 Antibody (D-7): m-IgG1 BP-HRP Bundle | sc-544498 | 200 µg Ab; 20 µg BP | $361.00 | |||
KLHL20 Antibody (D-7) AC | sc-515381 AC | 500 µg/ml, 25% agarose | $424.00 | |||
KLHL20 Antibody (D-7) HRP | sc-515381 HRP | 200 µg/ml | $322.00 | |||
KLHL20 Antibody (D-7) FITC | sc-515381 FITC | 200 µg/ml | $336.00 | |||
KLHL20 Antibody (D-7) PE | sc-515381 PE | 200 µg/ml | $349.00 | |||
KLHL20 Antibody (D-7) Alexa Fluor® 488 | sc-515381 AF488 | 200 µg/ml | $364.00 | |||
KLHL20 Antibody (D-7) Alexa Fluor® 546 | sc-515381 AF546 | 200 µg/ml | $364.00 | |||
KLHL20 Antibody (D-7) Alexa Fluor® 594 | sc-515381 AF594 | 200 µg/ml | $364.00 | |||
KLHL20 Antibody (D-7) Alexa Fluor® 647 | sc-515381 AF647 | 200 µg/ml | $364.00 | |||
KLHL20 Antibody (D-7) Alexa Fluor® 680 | sc-515381 AF680 | 200 µg/ml | $364.00 | |||
KLHL20 Antibody (D-7) Alexa Fluor® 790 | sc-515381 AF790 | 200 µg/ml | $364.00 |