Kininogen HC Antibody (1.B.708): sc-59580

Kininogen HC Antibody (1.B.708) is a mouse monoclonal IgG1 antibody that detects Kininogen HC in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Kininogen HC, a crucial component of the coagulation cascade, is a 644 amino acid precursor protein expressed by the KNG1 gene and secreted into blood plasma. Anti-Kininogen HC antibody (1.B.708) plays a vital role in studying blood coagulation by detecting proteins that ensure proper positioning of prekallikrein and Factor XI for interaction with Factor XII, which is essential for the activation of the coagulation pathway. Additionally, Kininogen HC is responsible for the release of bradykinin, a potent peptide that mediates various physiological responses, including smooth muscle contraction, induction of hypotension, regulation of blood glucose levels, and stimulation of nociceptors, thereby contributing to the inflammatory response. The ability of Kininogen HC to interact with these critical factors underscores its importance in maintaining hemostasis and regulating vascular functions. In contrast, Kininogen LC, another derivative of the Kininogen precursor, primarily inhibits thrombocyte aggregation and acts as a strong inhibitor of cysteine proteinases, highlighting the diverse functional roles of Kininogen proteins in the body. Kininogen HC monoclonal antibody (1.B.708) serves as an invaluable tool for researchers studying coagulation processes and related pathologies.

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Kininogen HC Antibody (1.B.708) References:

1. Platelet glycoprotein Ib: a zinc-dependent binding protein for the heavy chain of high-molecular-weight kininogen.  |  Joseph, K., et al. 1999. Mol Med. 5: 555-63. PMID: 10501658
2. Mapping of the H-kininogen binding site exposed by the prekallikrein heavy chain.  |  Herwald, H., et al. 1992. Agents Actions Suppl. 38 (Pt 1): 225-32. PMID: 1466274
3. Naturally occurring human antibodies against two distinct functional domains in the heavy chain of FXI/FXIa.  |  De La Cadena, RA., et al. 1988. Blood. 72: 1748-54. PMID: 2460161
4. Interaction of human calpains I and II with high molecular weight and low molecular weight kininogens and their heavy chain: mechanism of interaction and the role of divalent cations.  |  Ishiguro, H., et al. 1987. Biochemistry. 26: 2863-70. PMID: 3038169
5. A mechanism for hereditary angioedema with normal C1 inhibitor: an inhibitory regulatory role for the factor XII heavy chain.  |  Ivanov, I., et al. 2019. Blood. 133: 1152-1163. PMID: 30591525
6. Heavy chain of human high molecular weight and low molecular weight kininogens binds calcium ion.  |  Higashiyama, S., et al. 1987. Biochemistry. 26: 7450-8. PMID: 3427086
7. Human high molecular weight kininogen as a thiol proteinase inhibitor: presence of the entire inhibition capacity in the native form of heavy chain.  |  Higashiyama, S., et al. 1986. Biochemistry. 25: 1669-75. PMID: 3635411
8. Isolation and immunologic properties of a heterogeneous antigen with the characteristics of the heavy chain of human plasma kininogen.  |  Kärkkäinen, T., et al. 1982. Mol Immunol. 19: 179-89. PMID: 7043244
9. Localization of the binding site on plasma kallikrein for high-molecular-weight kininogen to both apple 1 and apple 4 domains of the heavy chain.  |  Page, JD., et al. 1994. Arch Biochem Biophys. 314: 159-64. PMID: 7944388
10. Thrombin-induced platelet aggregation is inhibited by the heptapeptide Leu271-Ala277 of domain 3 in the heavy chain of high molecular weight kininogen.  |  Kunapuli, SP., et al. 1996. J Biol Chem. 271: 11228-35. PMID: 8626672

The preferred Kininogen LC antibody is Kininogen LC Antibody (C11C1): sc-23915.