KHS Antibody (E-5): sc-374070
- KHS Antibody (E-5) is a mouse monoclonal IgG2a κ KHS antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-29 at the N-terminus of KHS of human origin
- KHS Antibody (E-5) is recommended for detection of KHS of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine
- Anti-KHS Antibody (E-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for KHS Antibody (E-5) for WB and IHC(P) applications. These reagents are now offered in bundles with KHS Antibody (E-5) (see ordering information below).
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KHS Antibody (E-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects KHS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-KHS antibody (E-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. KHS protein, known as kinase homologous to SPS1/STE20, plays a crucial role in cellular signaling pathways, particularly in the activation of stress response mechanisms. KHS (E-5) monoclonal antibody is closely related to the GC kinase and has been shown to activate several substrates, including c-Jun N-terminal kinase (JNK), which is vital for mediating cellular responses to stress stimuli. KHS protein′s ability to relay signals from G-protein coupled receptors to downstream MAP kinase cascades underscores its importance in regulating various physiological processes, including cell proliferation, differentiation, and apoptosis. Anti-KHS antibody (E-5) enables valuable insights into the functional dynamics of this kinase and its implications in cellular stress responses across different species.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
KHS Antibody (E-5) References:
- The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components. | Leberer, E., et al. 1992. EMBO J. 11: 4815-24. PMID: 1464311
- Molecular characterization of Ste20p, a potential mitogen-activated protein or extracellular signal-regulated kinase kinase (MEK) kinase kinase from Saccharomyces cerevisiae. | Wu, C., et al. 1995. J Biol Chem. 270: 15984-92. PMID: 7608157
- A novel human SPS1/STE20 homologue, KHS, activates Jun N-terminal kinase. | Tung, RM. and Blenis, J. 1997. Oncogene. 14: 653-9. PMID: 9038372
- NIK is a new Ste20-related kinase that binds NCK and MEKK1 and activates the SAPK/JNK cascade via a conserved regulatory domain. | Su, YC., et al. 1997. EMBO J. 16: 1279-90. PMID: 9135144
- Activation of the c-Jun N-terminal kinase pathway by a novel protein kinase related to human germinal center kinase. | Diener, K., et al. 1997. Proc Natl Acad Sci U S A. 94: 9687-92. PMID: 9275185
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KHS Antibody (E-5) | sc-374070 | 200 µg/ml | $316.00 | |||
KHS Antibody (E-5): m-IgG Fc BP-HRP Bundle | sc-529650 | 200 µg Ab; 10 µg BP | $354.00 | |||
KHS Antibody (E-5): m-IgGκ BP-HRP Bundle | sc-522607 | 200 µg Ab, 40 µg BP | $354.00 | |||
KHS Antibody (E-5): m-IgG2a BP-HRP Bundle | sc-547350 | 200 µg Ab; 10 µg BP | $354.00 | |||
KHS Antibody (E-5) AC | sc-374070 AC | 500 µg/ml, 25% agarose | $416.00 | |||
KHS Antibody (E-5) HRP | sc-374070 HRP | 200 µg/ml | $316.00 | |||
KHS Antibody (E-5) FITC | sc-374070 FITC | 200 µg/ml | $330.00 | |||
KHS Antibody (E-5) PE | sc-374070 PE | 200 µg/ml | $343.00 | |||
KHS Antibody (E-5) Alexa Fluor® 488 | sc-374070 AF488 | 200 µg/ml | $357.00 | |||
KHS Antibody (E-5) Alexa Fluor® 546 | sc-374070 AF546 | 200 µg/ml | $357.00 | |||
KHS Antibody (E-5) Alexa Fluor® 594 | sc-374070 AF594 | 200 µg/ml | $357.00 | |||
KHS Antibody (E-5) Alexa Fluor® 647 | sc-374070 AF647 | 200 µg/ml | $357.00 | |||
KHS Antibody (E-5) Alexa Fluor® 680 | sc-374070 AF680 | 200 µg/ml | $357.00 | |||
KHS Antibody (E-5) Alexa Fluor® 790 | sc-374070 AF790 | 200 µg/ml | $357.00 | |||
KHS (E-5) Neutralizing Peptide | sc-374070 P | 100 µg/0.5 ml | $68.00 |