Keap1 Antibody (G-2): sc-365626

Keap1 Antibody (G-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects Keap1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Keap1 antibody (G-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Keap1, also known as kelch-like ECH-associated protein 1, plays a crucial role as a stress-sensing adaptor in the Cullin3 (Cul3)-dependent E3 ubiquitin ligase complex, which is essential for the regulation of Nrf2 (NF-E2-related factor 2). This regulation is vital because Nrf2 is a key transcription factor that mediates the expression of cytoprotective genes in response to oxidative stress and electrophilic insults. Under normal conditions, Keap1 maintains low levels of Nrf2 by promoting ubiquitination and subsequent degradation, thereby preventing excessive activation of protective genes. During oxidative stress, modifications to specific cysteine residues in Keap1, particularly Cys 273 and Cys 288, lead to a conformational change that releases Nrf2, allowing nuclear translocation and target gene activation. This dynamic interplay between Keap1 and Nrf2 is critical for cellular defense mechanisms, making Keap1 an important protein in the study of oxidative stress responses and potential therapeutic targets for diseases related to oxidative damage.

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Keap1 Antibody (G-2) References:

1. Distinct cysteine residues in Keap1 are required for Keap1-dependent ubiquitination of Nrf2 and for stabilization of Nrf2 by chemopreventive agents and oxidative stress.  |  Zhang, DD. and Hannink, M. 2003. Mol Cell Biol. 23: 8137-51. PMID: 14585973
2. Oxidative stress sensor Keap1 functions as an adaptor for Cul3-based E3 ligase to regulate proteasomal degradation of Nrf2.  |  Kobayashi, A., et al. 2004. Mol Cell Biol. 24: 7130-9. PMID: 15282312
3. Keap1 is a redox-regulated substrate adaptor protein for a Cul3-dependent ubiquitin ligase complex.  |  Zhang, DD., et al. 2004. Mol Cell Biol. 24: 10941-53. PMID: 15572695
4. Utility of siRNA against Keap1 as a strategy to stimulate a cancer chemopreventive phenotype.  |  Devling, TW., et al. 2005. Proc Natl Acad Sci U S A. 102: 7280-7285A. PMID: 15883370
5. Keap1 regulates the oxidation-sensitive shuttling of Nrf2 into and out of the nucleus via a Crm1-dependent nuclear export mechanism.  |  Velichkova, M. and Hasson, T. 2005. Mol Cell Biol. 25: 4501-13. PMID: 15899855
6. Differential responses of the Nrf2-Keap1 system to laminar and oscillatory shear stresses in endothelial cells.  |  Hosoya, T., et al. 2005. J Biol Chem. 280: 27244-50. PMID: 15917255
7. Specific patterns of electrophile adduction trigger Keap1 ubiquitination and Nrf2 activation.  |  Hong, F., et al. 2005. J Biol Chem. 280: 31768-75. PMID: 15985429
8. Nrf2 controls constitutive and inducible expression of ARE-driven genes through a dynamic pathway involving nucleocytoplasmic shuttling by Keap1.  |  Nguyen, T., et al. 2005. J Biol Chem. 280: 32485-92. PMID: 16000310
9. The KEAP1-NRF2 System: a Thiol-Based Sensor-Effector Apparatus for Maintaining Redox Homeostasis.  |  Yamamoto, M., et al. 2018. Physiol Rev. 98: 1169-1203. PMID: 29717933
10. Polydatin prevents fructose-induced liver inflammation and lipid deposition through increasing miR-200a to regulate Keap1/Nrf2 pathway.  |  Zhao, XJ., et al. 2018. Redox Biol. 18: 124-137. PMID: 30014902
11. The Anti-Inflammatory and Anti-Oxidant Mechanisms of the Keap1/Nrf2/ARE Signaling Pathway in Chronic Diseases.  |  Tu, W., et al. 2019. Aging Dis. 10: 637-651. PMID: 31165007
12. A small-molecule inhibitor of Keap1-Nrf2 interaction attenuates sepsis by selectively augmenting the antibacterial defence of macrophages at infection sites.  |  Wang, Y., et al. 2023. EBioMedicine. 90: 104480. PMID: 36863256

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Keap1 Antibody (G-2) has 90 citations in a variety of scientific publications.

Primary antibodies like Anti-Keap1 Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Keap1 Antibody (G-2) Specifications

Alternate Product Name: Keap1

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 181-370

Epitope Target: Keap1

Epitope Species: human

Product Reactivity/Detection: Keap1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 90 citations in various scientific publications

Available Conjugates/Forms for Keap1 Antibody (G-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Keap1 antibody is Keap1 Antibody (F-10): sc-514914.