Keap1 Antibody (F-10): sc-514914

Keap1 Antibody (F-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects Keap1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Keap1 antibody (F-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Keap1, or kelch-like ECH-associated protein 1, plays a crucial role as a stress-sensing adaptor for the Cullin3-dependent E3 ubiquitin ligase complex, which is essential for the regulation of Nrf2 (NF-E2-related factor 2). The interaction between Keap1 and Nrf2 is vital for maintaining cellular homeostasis, as Keap1 negatively regulates Nrf2 under normal conditions, preventing Nrf2 accumulation in the nucleus. Upon exposure to oxidative stress or electrophiles, Keap1 undergoes conformational changes that lead to the release and subsequent activation of Nrf2, allowing Nrf2 to translocate to the nucleus and initiate the expression of cytoprotective genes. The cysteine residues at positions 273 and 288 of Keap1 are critical for suppressing Nrf2 nuclear accumulation, highlighting the importance of post-translational modifications in the regulation of this pathway. Understanding the balance maintained by the Keap1-Nrf2 system enables exploration of therapeutic strategies aimed at enhancing cellular defense mechanisms against oxidative damage.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Keap1 Antibody (F-10) has 33 citations in a variety of scientific publications.

Primary antibodies like Anti-Keap1 Antibody (F-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Keap1 Antibody (F-10) Specifications

Alternate Product Name: Keap1

Product Clone Name: F-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 39-65

Epitope Target: Keap1

Epitope Species: human

Product Reactivity/Detection: Keap1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 33 citations in various scientific publications

Available Conjugates/Forms for Keap1 Antibody (F-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Keap1 antibody is Keap1 Antibody (G-2): sc-365626.