IRSp53 Antibody (46): sc-136470

IRSp53 Antibody (46) is a mouse monoclonal IgG2a kappa light chain antibody that detects IRSp53 protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). Anti-IRSp53 antibody (46) is available in both non-conjugated and various conjugated forms, including agarose and horseradish peroxidase (HRP). The insulin receptor tyrosine kinase substrate p53 (IRSp53) is a crucial scaffolding protein that plays a significant role in regulating the actin cytoskeleton, particularly in mediating filopodia formation under the influence of Rho-family GTPases. IRSp53 is predominantly located in the cytoplasm, where IRSp53 serves as a vital link between small membrane-bound G-proteins and cytoplasmic effector proteins, facilitating cellular signaling pathways essential for cell motility and morphology. Structurally, IRSp53 features a central SH3 domain that interacts with proline-rich regions of various actin regulators, alongside a conserved N-terminal IRSp53/MIM homology domain (IMD) that exhibits F-actin-bundling activity. IRSp53 interacts with several key proteins, including atrophin-1, which is associated with dentatorubral-pallidoluysian atrophy, as well as ENAH, BAI-1, Eps8, and the Shank family of proteins (Shank 1, Shank 2, Shank 3), along with WAVE1, WAVE2, Tiam1, and Dia 1. These interactions underscore IRSp53′s importance in maintaining cellular architecture and signaling, making IRSp53 monoclonal antibody (46) an invaluable tool for researchers studying cytoskeletal dynamics and related pathologies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

IRSp53 Antibody (46) References:

1. Dentatorubral-pallidoluysian atrophy protein interacts through a proline-rich region near polyglutamine with the SH3 domain of an insulin receptor tyrosine kinase substrate.  |  Okamura-Oho, Y., et al. 1999. Hum Mol Genet. 8: 947-57. PMID: 10332026
2. The insulin receptor substrate IRSp53 links postsynaptic shank1 to the small G-protein cdc42.  |  Soltau, M., et al. 2002. Mol Cell Neurosci. 21: 575-83. PMID: 12504591
3. Genomic structure and alternative splicing of the insulin receptor tyrosine kinase substrate of 53-kDa protein.  |  Miyahara, A., et al. 2003. J Hum Genet. 48: 410-414. PMID: 12884081
4. IRSp53/Eps8 complex is important for positive regulation of Rac and cancer cell motility/invasiveness.  |  Funato, Y., et al. 2004. Cancer Res. 64: 5237-44. PMID: 15289329
5. Structural basis of filopodia formation induced by the IRSp53/MIM homology domain of human IRSp53.  |  Millard, TH., et al. 2005. EMBO J. 24: 240-50. PMID: 15635447
6. Regulation of dendritic spine morphogenesis by insulin receptor substrate 53, a downstream effector of Rac1 and Cdc42 small GTPases.  |  Choi, J., et al. 2005. J Neurosci. 25: 869-79. PMID: 15673667
7. NMDA receptor-dependent synaptic translocation of insulin receptor substrate p53 via protein kinase C signaling.  |  Hori, K., et al. 2005. J Neurosci. 25: 2670-81. PMID: 15758177
8. Tiam1-IRSp53 complex formation directs specificity of rac-mediated actin cytoskeleton regulation.  |  Connolly, BA., et al. 2005. Mol Cell Biol. 25: 4602-14. PMID: 15899863
9. Novel brain 14-3-3 interacting proteins involved in neurodegenerative disease.  |  Mackie, S. and Aitken, A. 2005. FEBS J. 272: 4202-10. PMID: 16098201
10. IRSp53/BAIAP2 in dendritic spine development, NMDA receptor regulation, and psychiatric disorders.  |  Kang, J., et al. 2016. Neuropharmacology. 100: 27-39. PMID: 26275848
11. IRSp53 promotes postsynaptic density formation and actin filament bundling.  |  Feng, Z., et al. 2022. J Cell Biol. 221: PMID: 35819332
12. Tunnelling nanotube formation is driven by Eps8/IRSp53-dependent linear actin polymerization.  |  Henderson, JM., et al. 2023. EMBO J. 42: e113761. PMID: 38009333

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IRSp53 Antibody (46) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-IRSp53 Antibody (46) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IRSp53 Antibody (46) Specifications

Alternate Product Name: IRSp53

Product Clone Name: 46

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 200-322

Epitope Target: IRSp53

Epitope Species: human

Product Reactivity/Detection: IRSp53

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for IRSp53 Antibody (46): AC: Agarose, HRP: Horseradish Peroxidase