IRS-4 Antibody (RI-7) recommended for detection of IRS-4 of human origin by WB, IP and ELISA

IRS-4 Antibody (RI-7): sc-100854

IRS-4 Antibody (RI-7) is rated 4.5 out of 5 by 4.
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Datasheets

    • IRS-4 Antibody (RI-7) is a mouse monoclonal IgG1 (kappa light chain) provided at 100 µg/ml
    • raised against recombinant IRS-4 of human origin
    • recommended for detection of IRS-4 of human origin by WB, IP and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IRS-4 (RI-7): sc-100854.

Which IRS4 epitope is recognized by the antibody? Is it N-terminal within the amino acids 3-342? Thank you.

Asked by: thegut
Thank you for your question. The exact epitope used to raise this monoclonal antibody has not been mapped so we are unable to provide this information. Please contact our Technical Service Department for further support. We are happy to help perform sequence analysis for you to answer any specific questions you have about the antibody reactivity.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-30

For Western Blot, is it recommended to use denatured or non-denatured conditions with IRS-4 (RI-7): sc-100854 antibody?

Asked by: Trav11
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 4 out of 5 by from Clean band in WB indicating a good antibody Clean band in WB indicating a good antibody to use especially in HeLa whole cell lysate. -SCBT QC
Date published: 2015-03-03
Rated 5 out of 5 by from Sizable band obtained in human IRS Sizable band obtained in human IRS-4 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-01-19
Rated 4 out of 5 by from Good publishable data on Western blot on Good publishable data on Western blot on human endothelial cells. -SCBT Publication Review
Date published: 2015-01-16
Rated 5 out of 5 by from Publishable Western blot and IP data using Publishable Western blot and IP data using human and mouse embryonic kidney epithelial 293T cells and NIH 3T3s -SCBT Publication Review
Date published: 2013-10-05
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