Insulin Antibody (2D11-H5): sc-8033

Insulin Antibody (2D11-H5) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect insulin across multiple species, including mouse, rat, human, bovine, and porcine, through IP, IF, IHCP, and ELISA applications. Insulin mouse monoclonal antibody (2D11-H5) targets an epitope comprising amino acids 1-84 of full-length porcine insulin, ensuring robust and reliable binding. Insulin, a crucial peptide hormone synthesized by the pancreas, plays a vital role in regulating blood glucose levels by facilitating glucose uptake and glycogen synthesis in cells, thereby decreasing blood glucose concentrations. Insulin is initially produced as preproinsulin, which is subsequently processed to proinsulin and finally to mature insulin, consisting of 51 amino acids divided into an A chain and a B chain connected by two disulfide bridges. Beyond glucose metabolism, insulin enhances cell permeability to monosaccharides, amino acids, and fatty acids, making insulin essential for overall cellular function and energy balance. The secretion of insulin is tightly regulated, with basal levels maintained in the absence of external stimuli and increased secretion in response to elevated blood glucose levels. Insulin exerts effects by binding to specific receptors on target cell membranes, initiating a cascade of intracellular events that promote metabolic activities. Dysregulation of insulin production or signaling can lead to conditions such as hyperproinsulinemia and type 2 diabetes mellitus, highlighting insulin′s importance in both research and clinical settings. Insulin mouse monoclonal antibody (2D11-H5) is recommended for researchers investigating insulin′s multifaceted roles in metabolism, mechanisms of action, and pathological implications of dysregulation. Insulin monoclonal antibody (2D11-H5) is available in both non-conjugated form and various conjugated forms, including agarose, HRP, PE, FITC, and multiple Alexa Fluor® conjugates.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Insulin Antibody (2D11-H5) References:

1. Stevioside does not cause increased basal insulin secretion or beta-cell desensitization as does the sulphonylurea, glibenclamide: studies in vitro.  |  Chen, J., et al. 2006. Life Sci. 78: 1748-53. PMID: 16260001
2. Dynamic strength training improves insulin sensitivity and functional balance between adrenergic alpha 2A and beta pathways in subcutaneous adipose tissue of obese subjects.  |  Polak, J., et al. 2005. Diabetologia. 48: 2631-40. PMID: 16273345
3. A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA.  |  Hilgert, I., et al. 1991. Hybridoma. 10: 379-86. PMID: 1916848
4. Differential signalling potential of insulin- and IGF-1-receptor cytoplasmic domains.  |  Lammers, R., et al. 1989. EMBO J. 8: 1369-75. PMID: 2548842
5. The molecular mechanism of insulin action.  |  Kahn, CR. 1985. Annu Rev Med. 36: 429-51. PMID: 2986528
6. Solution structure of the superactive monomeric des-[Phe(B25)] human insulin mutant: elucidation of the structural basis for the monomerization of des-[Phe(B25)] insulin and the dimerization of native insulin.  |  Jørgensen, AM., et al. 1996. J Mol Biol. 257: 684-99. PMID: 8648633
7. Cytosolic calcium oscillations and insulin release in pancreatic islets of Langerhans.  |  Soria, B. and Martin, F. 1998. Diabetes Metab. 24: 37-40. PMID: 9534007
8. Proinsulin: recent observations and controversies.  |  Mackin, RB. 1998. Cell Mol Life Sci. 54: 696-702. PMID: 9711235
9. Impaired beta cell glucose sensitivity and whole-body insulin sensitivity as predictors of hyperglycaemia in non-diabetic subjects.  |  Walker, M., et al. 2005. Diabetologia. 48: 2470-6. PMID: 16261308

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Insulin Antibody (2D11-H5) has 78 citations in a variety of scientific publications.

Primary antibodies like Anti-Insulin Antibody (2D11-H5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Insulin Antibody (2D11-H5) Specifications

Alternate Product Name: Insulin

Product Clone Name: 2D11-H5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-84

Epitope Target: Insulin

Epitope Species: porcine

Product Reactivity/Detection: Insulin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 78 citations in various scientific publications

Available Conjugates/Forms for Insulin Antibody (2D11-H5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Insulin B antibody is Insulin B Antibody (C-12): sc-377071.