Date published: 2025-9-5

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Insulin B 抗体 (C-12): sc-377071

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说明书
  • Insulin B 抗体 C-12 是小鼠单克隆 IgG1 κ,Insulin B抗体, 在23篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Insulin B的N-terminus附近的氨基酸33-69之间
  • Insulin B 抗体 (C-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Insulin B chain; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 抗Insulin B抗体(C-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体,可用于WB、IHC(P)和ELISA实验
  • 关于如何获取Insulin B (C-12): sc-377071免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Insulin B 抗体 (C-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Insulin B 抗体 (C-12) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

胰岛素B抗体(C-12)是一种IgG1 κ小鼠单克隆胰岛素B抗体(也称为胰岛素肽抗体、胰岛素生长激素抗体),它通过WB、IP、IF、IHC(P)和ELISA等方法检测小鼠、大鼠和人类来源的胰岛素B蛋白。胰岛素B抗体(C-12)既可作为非偶联的抗胰岛素B抗体形式提供,也可作为多种偶联形式的抗胰岛素B抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。胰岛素是一种分泌的肽类激素,具有引起代谢效应的作用,如增加葡萄糖摄取和糖原合成,导致血糖浓度降低。胰岛素最初以前体分子、前胰岛素原的形式形成,后来裂解为胰岛素原,最后裂解为成熟的胰岛素激素。成熟胰岛素由51个氨基酸组成,包含在一个A链和一个B链中,这两个链通过两个二硫桥连接。它能增加细胞对单糖、氨基酸和脂肪酸的通透性。在没有外源性刺激的情况下,胰腺会分泌基础水平的胰岛素,而胰岛素的分泌会随着葡萄糖的增加而增加。胰岛素的作用是通过胰岛素与靶细胞膜上的细胞表面受体结合而实现的。胰岛素的缺陷是高胰岛素原血症和2型糖尿病的原因。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Insulin B 抗体 (C-12) 参考文献:

  1. 胰岛素受体和 IGF-1 受体胞质结构域的信号潜力差异。  |  Lammers, R., et al. 1989. EMBO J. 8: 1369-75. PMID: 2548842
  2. 胰岛素作用的分子机制。  |  Kahn, CR. 1985. Annu Rev Med. 36: 429-51. PMID: 2986528
  3. 超活性单体 des-[Phe(B25)] 人胰岛素突变体的溶液结构:阐明 des-[Phe(B25)] 胰岛素单体化和原生胰岛素二聚化的结构基础。  |  Jørgensen, AM., et al. 1996. J Mol Biol. 257: 684-99. PMID: 8648633
  4. 朗格汉斯胰岛细胞膜钙振荡与胰岛素释放  |  Soria, B. and Martin, F. 1998. Diabetes Metab. 24: 37-40. PMID: 9534007
  5. 原胰岛素:最新观察和争议。  |  Mackin, RB. 1998. Cell Mol Life Sci. 54: 696-702. PMID: 9711235

订购信息

产品名称产品编号规格价格数量收藏夹

Insulin B 抗体 (C-12)

sc-377071
200 µg/ml
$316.00

Insulin B (C-12): m-IgG Fc BP-HRP 套装

sc-529966
200 µg Ab; 10 µg BP
$354.00

Insulin B (C-12): m-IgGκ BP-HRP 套装

sc-523173
200 µg Ab, 40 µg BP
$354.00

Insulin B (C-12): m-IgG1 BP-HRP 套装

sc-543793
200 µg Ab; 20 µg BP
$354.00

Insulin B 抗体 (C-12) AC

sc-377071 AC
500 µg/ml, 25% agarose
$416.00

Insulin B 抗体 (C-12) HRP

sc-377071 HRP
200 µg/ml
$316.00

Insulin B 抗体 (C-12) FITC

sc-377071 FITC
200 µg/ml
$330.00

Insulin B 抗体 (C-12) PE

sc-377071 PE
200 µg/ml
$343.00

Insulin B 抗体 (C-12) Alexa Fluor® 488

sc-377071 AF488
200 µg/ml
$357.00

Insulin B 抗体 (C-12) Alexa Fluor® 546

sc-377071 AF546
200 µg/ml
$357.00

Insulin B 抗体 (C-12) Alexa Fluor® 594

sc-377071 AF594
200 µg/ml
$357.00

Insulin B 抗体 (C-12) Alexa Fluor® 647

sc-377071 AF647
200 µg/ml
$357.00

Insulin B 抗体 (C-12) Alexa Fluor® 680

sc-377071 AF680
200 µg/ml
$357.00

Insulin B 抗体 (C-12) Alexa Fluor® 790

sc-377071 AF790
200 µg/ml
$357.00

Insulin B 抗体 (C-12) B

sc-377071 B
200 µg/ml
$320.00

Insulin B (C-12) 中和勝肽

sc-377071 P
100 µg/0.5 ml
$68.00

The molucular weight of insulin is 5808 Da, why the target band of this antibody is about 20 Kda?

Asked by: Ling
Thank you for your question. Please contact technical service by email scbt@scbt.com or call (800)-457-3801.
Answered by: BlakeJ
Date published: 2023-09-25

Can I use SC-377071 for sandwich ELISA?

Asked by: marthatyas
Thank you for your question. This antibody, sc-377071 Insulin B (C-12), has not been tested for sandwich ELISA, but it is recommended and warrantied for direct or indirect ELISA.
Answered by: Tech Service
Date published: 2019-09-18

Have you test this antibody through Native-Page Western Blot? Thanks

Asked by: marthatyas
Thank you for your question. No, this antibody has not been tested for Native-Page Western blot.
Answered by: Tech Service
Date published: 2019-09-15

I have contacted SCBT south east asia branch roughly 2 weeks a go to enquire about the 10 ug sample free of charge as stated in the product information Insulin B (C-12): sc-377071. However, there is no response. Can you help me regarding this matter?

Asked by: Agung
We are sorry to hear that you have not yet received a reply. If you are currently located in Indonesia, please contact one of our authorized distributors for information about how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-02-27

The expected molecular weight of Insulin is 6-7 kDa. Why 12 kDa? It´s not clear. Thank you.

Asked by: Jimena
Thank you for your question. The predicted molecular weight of 12 kDa is for the precursor of Insulin B which is 110 amino acids in length.
Answered by: Technical Support
Date published: 2023-10-04

what's the molecular weight of insulin (c-12)?

Asked by: weitang
Thank you for your question. Insulin B Antibody (C-12): sc-377071 is recommended for detection of Insulin B chain of mouse, rat and human origin. The expected molecular weight of Insulin B is 12 kDa.
Answered by: Technical Support
Date published: 2017-11-06

How can Insulin B Antibody (C-12): sc-377071 be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody is currently available conjugated to phycoerythrin (sc-377071 PE), fluorescein (sc-377071 FITC), Alexa Fluor® 488 (sc-377071 AF488) or Alexa Fluor® 647 (sc-377071 AF647).
Answered by: Technical Support 8
Date published: 2017-01-06
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Rated 5 out of 5 by from Excellent Signal!Just wanted to say how pleased I am with the antibody sample I was given! Great strong insulin signal on my mouse paraffin embedded sections for IF staining! Highly recommend!
Date published: 2017-09-13
Rated 4 out of 5 by from Primate retinaAntibody was tested under standard conditions (Long Y, Bordt AS, Liu WS, Davis EP, Lee SJ, Tseng L, Chuang AZ, Whitaker CM, Massey SC, Sherman MB, Marshak DW. Wide-field diffuse amacrine cells in the monkey retina contain immunoreactive Cocaine- and Amphetamine-Regulated Transcript (CART). Peptides. 2016 Oct;84:22-35). The isolated retina of a baboon was fixed for 60 minutes in 4% paraformaldehyde. Tissue was incubated for 9 days in a 1:1,000 solution of primary antibody with detergent and a blocking agent at 4 °C, washed, then incubated in an affinity-purified secondary antibody for 4 days at 4 °C, washed, and mounted in medium containing DAPI. No peptide blocking control was performed. Results: Very light punctate labeling and low background throughout the optic fiber layer, ganglion cell layer, inner plexiform layer, and inner nuclear layer (less).
Date published: 2017-06-01
Rated 5 out of 5 by from greatIn our experiment, we use this product testing, this has been working really well
Date published: 2017-05-17
Rated 5 out of 5 by from High antibody titerget this antibody two months ago,the antibody titer was high while doing immunofluorescence(1:200)
Date published: 2017-05-04
Rated 5 out of 5 by from specific staningThe results insulin mainly localizes to cytoplasm of embryonic cardiomyocytes. Patchy staining was also observed in nuclei.
Date published: 2017-01-29
Rated 5 out of 5 by from Superb resultsStrong results for both western blot and ihc applications
Date published: 2017-01-03
Rated 5 out of 5 by from Good Insulin antibodyA good choice for detecting Insulin B by Western or IHC. It has great specificity and will provide clean blots and stains with strong signal.
Date published: 2016-12-29
Rated 4 out of 5 by from Worked wellGot this as a sample antibody to try out and it worked well on our mouse pancreatic cell line.
Date published: 2016-11-29
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Insulin B 抗体 (C-12) is rated 4.8 out of 5 by 12.
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