IL-6 Antibody (10E5) recommended for detection of IL-6 of mouse and rat origin by WB, IP, FCM and ELISA

IL-6 Antibody (10E5): sc-57315

IL-6 Antibody (10E5) is rated 4.3 out of 5 by 27.
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Datasheets

    • IL-6 Antibody (10E5) is a mouse monoclonal IgG2b (kappa light chain) provided at 100 µg/ml
    • raised against full length IL-6 of rat origin
    • recommended for detection of IL-6 of mouse and rat origin by WB, IP, FCM and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IL-6 (10E5): sc-57315.
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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What is the molecular weight of IL-6 ?

Asked by: AbPolly
Thank you for your question. IL-6 has an expected molecular weight of approximately 21 kDa. If you have any questions, please contact our Technical Service Department and we would be happy to assist you.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 3 out of 5 by from It doesn't work in WB of secretome of C2C12 We have used this antibody to detect Il6 on conditioned medium of C2C12 cells at 1:1000 diluition and it doesn't work. It could be that the amount of IL6 produced by these cells is too low
Date published: 2018-10-05
Rated 5 out of 5 by from Works well in rat frozen liver sections Rat liver frozen sections were subjected to immunofluorescence using IL-6 (10E5) antibody. Antigen retrieval methods were optimized between without antigen retrieval, heat-induced citrate buffer, and trypsin-induced antigen retrieval. Positive signals in portal tracts were clear without any antigen retrieval.
Date published: 2018-03-14
Rated 5 out of 5 by from Work very well Have been using this antibody for Western blot detecting IL-6 in mouse tissue and C2C12 samples. Always got clear, specific band. would like to recommend it.
Date published: 2018-03-11
Rated 4 out of 5 by from Good! I got a small sample and tried it for WB in a dilution of 1:300. The results were great.
Date published: 2018-03-02
Rated 5 out of 5 by from good features work well in WB at 1:500, with hippocampus of mice.but, it has two bands, one is about 28kd,another is 20kd. So,which one is what i need?
Date published: 2018-02-01
Rated 5 out of 5 by from Skeletal muscle immunostaining Bright staining of nuclei in frozen skeletal muscle samples.
Date published: 2017-11-22
Rated 4 out of 5 by from Good antibody formalin fixed rat brain , IF staining, 1:100 dilution. got good result
Date published: 2017-10-24
Rated 3 out of 5 by from 总体效果一般 该抗体不能与人体组织反应,是一个重要的缺陷。在使用过程中,在动物组织中检测该蛋白的表达改变,需要适当增加上样量,提高1抗的浓度。
Date published: 2017-09-28
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