Anti-IL-6 Antibody (1) recommended for detection of IL-6 of human origin by WB, IF and IHC(P)

Anti-IL-6 Antibody (1): sc-130326

Anti-IL-6 Antibody (1) is rated 4.5 out of 5 by 11.
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Datasheets

    • IL-6 Antibody (1) is a mouse monoclonal IgG2b κ, cited in 63 publications, provided at 200 µg/ml
    • raised against recombinant IL-6 of human origin
    • IL-6 Antibody (1) is recommended for detection of IL-6 of human origin by WB, IF and IHC(P)
    • Anti-IL-6 Antibody (1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IL-6 (1): sc-130326.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for IL-6 Antibody (1) for WB and IHC(P) applications. These reagents are now offered in bundles with IL-6 Antibody (1) (see ordering information below).
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Can IL-6 Ab (sc-130326) be also used as a neutralising antibody in order to decoy IL-6? Thanks in advance

Asked by: Rihan7
Thank you for your question. We have not tested this antibody, IL-6 (1): sc-130326, for any biological activity including neutralization, and so this is not a recommended application at this time. As an alternative, we would recommend our mouse monoclonal, IL-6 (AH65): sc-53865, which is provided azide-free for neutralizing, sc-53865 L, 200 µg/0.1 ml. A link to the product information on our website for this antibody is provided below. https://www.scbt.com/scbt/product/il-6-antibody-ah65
Answered by: Technical Support
Date published: 2017-09-05

What is the total volume provided of IL-6 (1):sc-130326 monoclonal antibody?

Asked by: jenni
Thank you for your question. This antibody is provided as 200 µg in 1 ml total volume of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 13
Date published: 2017-01-13

Can IL-6 (1): sc-130326 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, IL-6 (1): sc-130326 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 13
Date published: 2017-01-13
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Rated 4 out of 5 by from 功能出色 之前申请的试用装,结果显示,该抗体能特异性识别相应抗原,染色结果较清晰,适用于免疫荧光实验及相关实验检测。
Date published: 2018-04-24
Rated 4 out of 5 by from Good antibody Worked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from In IHC Great monoclonal antibody, It works well on human lymph node tissue by IHC
Date published: 2017-08-16
Rated 5 out of 5 by from It's worked well Used 1:100 dilution for Immunoperoxidase analysis of paraffin embedded human lymph node tissue, good dyeing effect.
Date published: 2017-05-07
Rated 5 out of 5 by from staining Strong staining in IHC in human lymph node tissue, behave well!
Date published: 2017-04-22
Rated 5 out of 5 by from Great antibody Strong western blot and IHC results; better than polyclonals
Date published: 2017-01-20
Rated 5 out of 5 by from Good for IHC This IL-6 antibody shows great results for IHC staining of human tissue.
Date published: 2017-01-12
Rated 5 out of 5 by from great I used this antibody to detect the content of pre-muture IL-6 in the lysis collected from human macrophages THP-1.
Date published: 2016-12-05
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