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IKKγ Antibody (F-10): sc-166398

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Datasheets
  • IKKγ Antibody (F-10) is a mouse monoclonal IgG1 κ IKKγ antibody, cited in 5 publications, provided at 200 µg/ml
  • raised against full length IKKΓ of human origin
  • IKK gamma Antibody (F-10) is recommended for detection of IKKγ of mouse, rat and human origin by WB, IP and IF
  • Anti-IKK gamma Antibody (F-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for IKKγ Antibody (F-10) for WB applications. These reagents are now offered in bundles with IKKγ Antibody (F-10) (see ordering information below).

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IKKγ Antibody (F-10) is a mouse monoclonal IgG1 kappa kappa light chain antibody designed to detect IKKγ protein, alternatively referred to as NF-κB essential modulator, or NEMO, across human, mouse, and rat species. Anti-IKKγ antibody (F-10) targets the full-length human IKKγ epitope and is suitable for applications including western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). IKKγ plays a pivotal role as a regulatory subunit within the IκB kinase (IKK) complex, which is essential for the activation of the NF-κB pathway, a key regulator of immune response, inflammation, and cell survival. By facilitating the phosphorylation and subsequent degradation of IκB proteins, IKKγ enables the translocation of NF-κB into the nucleus, where IKKγ can initiate transcription of target genes involved in various physiological processes. Understanding and detecting IKKγ is crucial for research into inflammatory diseases, cancer, and immune disorders, making anti-IKKγ antibody (F-10) an essential resource for elucidating NF-κB signaling pathways and their role in disease progression, while offering high specificity and reliability across various experimental applications. IKKγ monoclonal antibody (F-10) is available in both non-conjugated form and several conjugated variants, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing flexibility for diverse experimental needs.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

IKKγ Antibody (F-10)

sc-166398
200 µg/ml
$316.00

IKKγ Antibody (F-10): m-IgG Fc BP-HRP Bundle

sc-528955
200 µg Ab; 10 µg BP
$354.00

IKKγ Antibody (F-10): m-IgGκ BP-HRP Bundle

sc-521590
200 µg Ab, 40 µg BP
$354.00

IKKγ Antibody (F-10): m-IgG1 BP-HRP Bundle

sc-543234
200 µg Ab; 20 µg BP
$354.00

IKKγ Antibody (F-10) AC

sc-166398 AC
500 µg/ml, 25% agarose
$416.00

IKKγ Antibody (F-10) HRP

sc-166398 HRP
200 µg/ml
$316.00

IKKγ Antibody (F-10) FITC

sc-166398 FITC
200 µg/ml
$330.00

IKKγ Antibody (F-10) PE

sc-166398 PE
200 µg/ml
$343.00

IKKγ Antibody (F-10) Alexa Fluor® 488

sc-166398 AF488
200 µg/ml
$357.00

IKKγ Antibody (F-10) Alexa Fluor® 546

sc-166398 AF546
200 µg/ml
$357.00

IKKγ Antibody (F-10) Alexa Fluor® 594

sc-166398 AF594
200 µg/ml
$357.00

IKKγ Antibody (F-10) Alexa Fluor® 647

sc-166398 AF647
200 µg/ml
$357.00

IKKγ Antibody (F-10) Alexa Fluor® 680

sc-166398 AF680
200 µg/ml
$357.00

IKKγ Antibody (F-10) Alexa Fluor® 790

sc-166398 AF790
200 µg/ml
$357.00

Can IKKγ (F-10): sc-166398 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IKKγ (F-10): sc-166398 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good antibody for detecting endogenous NEMOExcellent antibody for detecting endogenous NEMO. It is also good for IP.
Date published: 2017-09-22
Rated 2 out of 5 by from MW band wrong size.I previously used a Santa Cruz anti-IKKg antibody and liked it very much. As it was discontinued, I tried this one instead. There is a faint band that is almost the right size (a bit low), and several strong bands of completely the wrong size.
Date published: 2017-03-07
Rated 5 out of 5 by from Works wellantibody works well in WB of lysis of MEFs/BMDM cells
Date published: 2016-12-02
Rated 5 out of 5 by from Highly potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was very strong detection of the ~48 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested.
Date published: 2016-11-10
Rated 5 out of 5 by from Fantastic WB data in mouse and human transfectedFantastic WB data in mouse and human transfected lysates. -SCBT QC
Date published: 2013-02-02
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IKKγ Antibody (F-10) is rated 4.4 out of 5 by 5.
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