IKK gamma Antibody (B-3) recommended for detection of IKKγ of mouse, rat and human origin by WB, IP, IF and ELISA

IKKγ Antibody (B-3): sc-8032

IKKγ Antibody (B-3) is rated 4.8 out of 5 by 9.
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Datasheets

    • IKKγ Antibody (B-3) is a mouse monoclonal IgG1 κ, cited in 33 publications, provided at 200 µg/ml
    • raised against amino acids 1-419 representing full length IKKγ of human origin
    • Anti-IKK gamma Antibody (B-3) is recommended for detection of IKKγ of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-IKK gamma Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IKKγ (B-3): sc-8032.

What is the dilution of SC-8032 for WB?

Asked by: zheny
Thank you for your question. We recommend a starting dilution of 1:200 (1:100-1:1,000) for Western blotting.
Answered by: Technical Support
Date published: 2019-07-13

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with IKKy Antibody (B-3): sc-8032?

Asked by: Trav11
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2017-01-30
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Rated 5 out of 5 by from Well characterized IKK gamma ab for WB, IF & IHC Produced more than a 2 dozen citations in the primarey literature on human, mouse and rat samples in a wide variety of applications.
Date published: 2017-01-06
Rated 5 out of 5 by from good This antibody is very good for WB in 293 and other human cells
Date published: 2016-12-05
Rated 5 out of 5 by from great this antibody is very good for Western blot in 293 cells and 549.
Date published: 2016-12-02
Rated 4 out of 5 by from IF images were good IF images were good. Worked well on mouse cells.
Date published: 2015-10-12
Rated 4 out of 5 by from Used in IP in mouse cells Used in IP in mouse cells.
Date published: 2015-07-29
Rated 5 out of 5 by from WB Published with this antibody using human WB Published with this antibody using human HEK293T. -SCBT Publication Review
Date published: 2015-05-14
Rated 5 out of 5 by from Exceptional immunofluorescence cytoplasmic Exceptional immunofluorescence cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-04-13
Rated 5 out of 5 by from Great antibody for human IP Great antibody for human IP, published using t-cells. -SCBT Publication Review
Date published: 2015-03-18
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