IKK alpha Antibody (D-5): sc-136978

IKKα Antibody (D-5) is a mouse monoclonal IgG2a antibody that detects IKKα in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). IKKα, also known as CHUK, plays a pivotal role in activating the transcription factor NFκB, which regulates immune responses and inflammation. Located primarily in the cytoplasm, IKKα interacts with the inhibitory protein IκB, facilitating phosphorylation on specific serine residues. This post-translational modification leads to IκB degradation, allowing NFκB to translocate to the nucleus and initiate transcription of target genes involved in inflammatory responses. IKKα′s importance in this signaling pathway is underscored by interaction with NFκB inducing kinase (NIK), which acts as a central regulator for NFκB activation in response to proinflammatory cytokines such as tumor necrosis factor (TNF) and interleukin-1 (IL-1). The functional IKK complex, comprising IKKα, IKKβ, and IKKγ (NEMO), is critical for IκB phosphorylation, highlighting essential contributions of each subunit in NFκB signaling regulation. Anti-IKKα antibody (D-5) serves as an invaluable tool for researchers studying inflammation and immune response mechanisms.

The preferred IKK alpha antibody is IKK alpha Antibody (B-8): sc-7606.