IKK alpha Antibody (B-8) recommended for detection of IKKα of human origin by WB, IP, IF, IHC(P), FCM and ELISA

IKKα Antibody (B-8): sc-7606

IKKα Antibody (B-8) is rated 4.7 out of 5 by 15.
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Datasheets

    • Anti-IKKα Antibody (B-8) is a mouse monoclonal IgG2b κ IKKα antibody, cited in 81 publications, provided at 200 µg/ml
    • raised against amino acids 1-745 representing full length IKKα of human origin
    • IKK alpha Antibody (B-8) is recommended for detection of IKKα of human origin by WB, IP, IF, IHC(P), FCM and ELISA
    • Anti-IKK alpha Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • TransCruz reagent for ChIP application (sc-7606 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IKKα (B-8): sc-7606.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for IKKα Antibody (B-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with IKKα Antibody (B-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

I would like to use this antibody to perform a double stain. Do you know how to do this with only mouse monoclonal primary antibodies?

Asked by: Cweed
We recommend using directly conjugated primary antibodies. This product, sc-7606, is available conjugated to FITC, AF488, and AF647.
Answered by: Technical Support 4
Date published: 2017-01-16
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Rated 4 out of 5 by from WB Good Western Blot results of I KK-alpha expression in Huh7 cells,1:300 dilution was used.
Date published: 2018-09-15
Rated 4 out of 5 by from not very specific for WB this antibody shows weak target band acompanies nonspecific bands in WB in human lung cencer cell lysates
Date published: 2017-08-02
Rated 5 out of 5 by from Very good antibody I used this antibody in my previous lab and got pretty good data. I am doing a pioneer experiment in new lab now with NFKB research, hope to get small mount antibody for it.
Date published: 2017-08-01
Rated 5 out of 5 by from recommend this antibody I had detected this antbody,I suggest loading 20ug sample, The antibody diluted 1:600 for WB analyisis for turbot intestine tissue .
Date published: 2017-07-18
Rated 5 out of 5 by from Good antibody. It works well in WB at dilution 1:500. I got clear band.
Date published: 2017-07-13
Rated 5 out of 5 by from Great We usually use 1:1000 for WB, It worked well.very nice
Date published: 2017-04-22
Rated 4 out of 5 by from GOOD RESULT Works well for WB. Tested at 1:1000. Happy with the antibody.
Date published: 2017-03-08
Rated 5 out of 5 by from Good for Western blot and IHC Strong band in Western blot (WB) at ~88 kDa. Works great for IHC(P) on human gall bladder tissue.
Date published: 2016-12-20
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