IgD Antibody (AMS 9.1) recommended for detection of Fc portion of mouse IgD of Igh-Ca haplotype of and mouse origin by WB, IP, IF and FCM

IgD Antibody (AMS 9.1): sc-53853

IgD Antibody (AMS 9.1) is rated 4.7 out of 5 by 3.
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Datasheets

    • IgD Antibody (AMS 9.1) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against BALB/c splenocytes of mouse origin
    • recommended for detection of Fc portion of mouse IgD of Igh-Ca haplotype of and mouse origin by WB, IP, IF and FCM; non cross-reactive with IgD of Igh-Cb or Igh-Ce haplotypes
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also provided azide-free for in vitro stimulation, sc-53853 L, 200 µg/0.1 ml
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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How can IgD (AMS 9.1): sc-53853 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: DefinitelyNotMatt
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-53853 PE) or fluoroscein (sc-53853 FITC).
Answered by: Technical Support
Date published: 2017-02-28
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Date published: 2015-03-09
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