IgD Antibody (IgD26) recommended for detection of IgD of human origin by WB, IP, IF, IHC(P) and FCM

IgD Antibody (IgD26): sc-53345

IgD Antibody (IgD26) is rated 5.0 out of 5 by 2.
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Datasheets

    • IgD Antibody (IgD26) is a mouse monoclonal IgG1 IgD antibody provided at 200 µg/ml
    • raised against IgD purified from human myeloma serum
    • IgD Antibody (IgD26) is recommended for detection of IgD of human origin by WB, IP, IF, IHC(P) and FCM
    • IgD Antibody (IgD26) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IgD (IgD26): sc-53345.
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用IgD与细胞结合后,用IgD(IgD26):sc53345作为一抗,做免疫荧光是否可以?

Asked by: 药理研究所
感谢您的提问。IgD Antibody (IgD26): sc-53345 推荐用于人源样本的IgD的免疫荧光检测。 Thank you for your question. IgD Antibody (IgD26): sc-53345 is recommended for detection of IgD of human origin by IF.
Answered by: Technical Support 8
Date published: 2018-12-21

Is this antibody IgG1κ or IgG1λ? I want to use m-IgGκ BP-PE (sc-516141) as secondary antibody but I don't know if it will work. Thank you for your time.

Asked by: Eva27
Thank you for your question. Unfortunately, we have not yet determined the type of light chain for this mouse monoclonal primary antibody, IgD (IgD26): sc-53345. We would recommend testing it with the m-IgGκ BP-PE (sc-516141) since the majority (>98%) of mouse IgG has a kappa light chain.
Answered by: Technical Support
Date published: 2017-09-08

Can IgD (IgD26): sc-53345 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IgD (IgD26): sc-53345 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Nice signal strength in WB against NAMALWA Nice signal strength in WB against NAMALWA whole cell lysate.
Date published: 2015-01-29
Rated 5 out of 5 by from Excellent indirect FCM data of human peripheral Excellent indirect FCM data of human peripheral blood leukocytes stained with IgD . -SCBT QC
Date published: 2014-05-07
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