IgD Antibody (IgD26) is a mouse monoclonal IgG1IgD antibody provided at 200 µg/ml
raised against IgD purified from human myeloma serum
IgD Antibody (IgD26) is recommended for detection of IgD of human origin by WB, IP, IF, IHC(P) and FCM
IgD Antibody (IgD26) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IgD (IgD26): sc-53345.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
IgD Antibody (IgD26) is a high quality monoclonal IgD antibody suitable for the detection of the IgD antigen. Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through interchain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin D (IgD) exists as a monomer with delta heavy chains and either kappa or lambda light chains. It plays a biological role as a transmembrane receptor molecule, co-expressed with IgM on the surface of mature/naive B cells. In particular, it is found on spleen B cell surfaces. Compared to IgM, IgD exists in much lower numbers and is not expressed on immature B cells. IgD surface expression on B cells is regulated in part by IL-27. In mice, the inhibition of this immunoglobulin isotype does not cause a significant change to the immune system.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
感谢您的提问。IgD Antibody (IgD26): sc-53345 推荐用于人源样本的IgD的免疫荧光检测。 Thank you for your question. IgD Antibody (IgD26): sc-53345 is recommended for detection of IgD of human origin by IF.
Answered by: Technical Support 8
Date published: 2018-12-21
Is this antibody IgG1κ or IgG1λ? I want to use m-IgGκ BP-PE (sc-516141) as secondary antibody but I don't know if it will work.
Thank you for your time.
Asked by: Eva27
Thank you for your question. Unfortunately, we have not yet determined the type of light chain for this mouse monoclonal primary antibody, IgD (IgD26): sc-53345. We would recommend testing it with the m-IgGκ BP-PE (sc-516141) since the majority (>98%) of mouse IgG has a kappa light chain.
Answered by: Technical Support
Date published: 2017-09-08
Can IgD (IgD26): sc-53345 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?
Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IgD (IgD26): sc-53345 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
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