IFN- gamma R alpha Antibody (GIR-94) recommended for detection of IFN-γRα of mouse, rat and human origin by WB, IP, IF and FCM

IFN-γRα Antibody (GIR-94): sc-12755

IFN-γRα Antibody (GIR-94) is rated 4.6 out of 5 by 7.
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Datasheets

    • Anti-IFN-γRα Antibody (GIR-94) is a mouse monoclonal IgG2b (kappa light chain) IFN-γRα antibody provided at 200 µg/ml
    • raised against purified IFN-γRα of human origin
    • Anti-IFN- gamma R alpha Antibody (GIR-94) is recommended for detection of IFN-γRα of mouse, rat and human origin by WB, IP, IF and FCM
    • Anti-IFN- gamma R alpha Antibody (GIR-94) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IFN-γRα (GIR-94): sc-12755.

Hi! Can sc-12755 be used for IHC staining? Also, is it membrane stained? Thanks in advance!

Asked by: XiaoJ
Thank you for your question. We have not yet tested this antibody, IFN-γRα Antibody (GIR-94): sc-12755, on formalin-fixed paraffin-embedded tissue sections, and so IHC(P) is not a recommended application at this time. However, we do recommend and warranty the antibody for IF, or immunofluorescent staining of fresh or frozen tissue sections or cells fixed in acetone or methanol. We do expect membrane staining for IFN-γRα. If you are looking for an antibody for IHC(P) in human tissues, we would recommend an alternative clone, IFN-γRα Antibody (D-3): sc-28363. A link to that product on our website is below. https://www.scbt.com/scbt/product/ifn-gammaralpha-antibody-d-3 (D-3): sc-28363.
Answered by: Technical Support
Date published: 2017-07-27

Can IFN-γRα (GIR-94): sc-12755 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IFN-γRα (GIR-94): sc-12755 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24

Hi, I am interested in using this antibody for Western Blot of mouse cells. However, I checked the same clone (GIR-94) from BD science, they said it is only used for human. Could you let me know if this antibody is suitable in detecting mouse IFNGR1?

Asked by: Mario
Thank you for your question. We have tested this mouse monoclonal, IFN-γRα Antibody (GIR-94): sc-12755, in mouse samples and have verified reactivity with mouse peripheral blood leukocytes in Flow Cytometry. Therefore, we do recommend this antibody for detection of mouse IFNGR1 by Western blotting as well.
Answered by: Technical Support
Date published: 2016-12-05
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Rated 5 out of 5 by from Tested on HFFF by FACS This antibody was tested on HFFF cells by FACS and shown a very strong signal, even at dilution 1:100
Date published: 2018-11-16
Rated 4 out of 5 by from Nice Results for WB Reproducible Results for Western blot using lysates from different cell lines.
Date published: 2017-11-06
Rated 5 out of 5 by from worked well with mouse cells by flow We got very good flow cytometry results in mouse ESCs and their differentiated fibroblasts with this antibody at 1:100
Date published: 2017-05-05
Rated 5 out of 5 by from published soon This antibody worked well with human IFN-gamma R alpha precursor transfected 293T whole cell lysate by WB. The data will be published soon.
Date published: 2016-12-18
Rated 5 out of 5 by from Good WB publishable data gathered with human Good WB publishable data gathered with human dermal lesion tissue. -SCBT Publication Review
Date published: 2015-05-25
Rated 3 out of 5 by from Large robust bands with background seen using Large robust bands with background seen using Raji and AML-193 whole cell lysates. -SCBT QC
Date published: 2015-01-05
Rated 5 out of 5 by from Great FCM data of mouse peripheral blood Great FCM data of mouse peripheral blood leukocytes. -SCBT QC
Date published: 2013-05-27
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