Id3 Antibody (2B11): sc-56712

Id3 Antibody (2B11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Id3 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Id3 (2B11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Id3, a member of the Id family of basic helix-loop-helix (bHLH) proteins, plays a crucial role in cellular differentiation and proliferation by acting as a negative regulator of DNA binding for bHLH proteins. Id3 can dimerize with class A and B HLH proteins, preventing these complexes from binding to DNA and inhibiting gene expression necessary for muscle differentiation. As muscle cells differentiate, Id protein levels decrease, allowing for active heterodimer formation that promotes myogenic differentiation. Growth factors tightly regulate Id3 expression, and studies show reduced Id mRNA levels can delay quiescent cells′ reentry into the cell cycle upon growth factor stimulation, highlighting Id3 ′s significance in developmental processes and potential implications in muscle-related pathologies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Id3 Antibody (2B11) References:

1. Id3, E47, and SREBP-1c: fat factors controlling adiponectin expression.  |  Rahmouni, K. and Sigmund, CD. 2008. Circ Res. 103: 565-7. PMID: 18796641
2. Id proteins Id1 and Id2 selectively inhibit DNA binding by one class of helix-loop-helix proteins.  |  Sun, XH., et al. 1991. Mol Cell Biol. 11: 5603-11. PMID: 1922066
3. An Id-related helix-loop-helix protein encoded by a growth factor-inducible gene.  |  Christy, BA., et al. 1991. Proc Natl Acad Sci U S A. 88: 1815-9. PMID: 2000388
4. The protein Id: a negative regulator of helix-loop-helix DNA binding proteins.  |  Benezra, R., et al. 1990. Cell. 61: 49-59. PMID: 2156629
5. CD5L Promotes M2 Macrophage Polarization through Autophagy-Mediated Upregulation of ID3.  |  Sanjurjo, L., et al. 2018. Front Immunol. 9: 480. PMID: 29593730
6. Tissue-infiltrating alloreactive T cells require Id3 to deflect PD-1-mediated immune suppression during GVHD.  |  Wang, Y., et al. 2024. Blood. 143: 166-177. PMID: 37871574
7. Investigating the ID3/SLC22A4 as immune-related signatures in ischemic stroke.  |  Lu, D., et al. 2023. Aging (Albany NY). 15: 14803-14829. PMID: 38112574
8. The nuclear factor ID3 endows macrophages with a potent anti-tumour activity.  |  Deng, Z., et al. 2024. Nature. 626: 864-873. PMID: 38326607
9. HLH forced dimers: tethering MyoD to E47 generates a dominant positive myogenic factor insulated from negative regulation by Id.  |  Neuhold, LA. and Wold, B. 1993. Cell. 74: 1033-42. PMID: 7691411
10. The expression pattern of Id4, a novel dominant negative helix-loop-helix protein, is distinct from Id1, Id2 and Id3.  |  Riechmann, V., et al. 1994. Nucleic Acids Res. 22: 749-55. PMID: 8139914
11. Id proteins control growth induction in mammalian cells.  |  Barone, MV., et al. 1994. Proc Natl Acad Sci U S A. 91: 4985-8. PMID: 8197168
12. Id-related genes encoding helix-loop-helix proteins are required for G1 progression and are repressed in senescent human fibroblasts.  |  Hara, E., et al. 1994. J Biol Chem. 269: 2139-45. PMID: 8294468

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Id3 Antibody (2B11) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-Id3 Antibody (2B11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Id3 Antibody (2B11) Specifications

Alternate Product Name: Id3

Product Clone Name: 2B11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-119

Epitope Target: Id3

Epitope Species: mouse

Product Reactivity/Detection: Id3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for Id3 Antibody (2B11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Id1 antibody is Id1 Antibody (B-8): sc-133104.