ICSBP Antibody (F-11): sc-398237

ICSBP Antibody (F-11) is a mouse monoclonal IgG1 antibody that detects ICSBP (Interferon consensus sequence-binding protein, also known as Interferon regulatory factor 8; IRF-8) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ICSBP plays a crucial role as a transcription factor in the regulation of immune responses, particularly in signaling pathways mediated by interferon-gamma (IFN-γ) during dendritic cell and macrophage differentiation. ICSBP (F-11) antibody helps researchers study ICSBP′s physical interaction with TRAF6, which is essential for modulating Toll-like receptor (TLR) signaling and facilitating cross-talk between IFN-γ and TLR pathways. ICSBP has been shown to antagonize the oncogenic Bcr/Abl fusion protein by downregulating Bcl-2, thereby influencing cell survival and apoptosis. Structurally, ICSBP belongs to the interferon regulatory factor (IRF) family, characterized by a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal regulatory domain, which allows binding to the interferon-stimulated response element (ISRE) and regulation of key cytokines such as IFN-α and IFN-β. ICSBP′s interaction with chromatin and binding to PU.1 in macrophages further highlights anti-ICSBP antibody (F-11)′s significance as a valuable tool for studying immune signaling and transcriptional regulation.

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ICSBP Antibody (F-11) References:

1. Interferon-gamma signaling in human retinal pigment epithelial cells mediated by STAT1, ICSBP, and IRF-1 transcription factors.  |  Li, W., et al. 1999. Invest Ophthalmol Vis Sci. 40: 976-82. PMID: 10102295
2. Gamma interferon triggers interaction between ICSBP (IRF-8) and TEL, recruiting the histone deacetylase HDAC3 to the interferon-responsive element.  |  Kuwata, T., et al. 2002. Mol Cell Biol. 22: 7439-48. PMID: 12370291
3. The interferon regulatory factor ICSBP/IRF-8 in combination with PU.1 up-regulates expression of tumor suppressor p15(Ink4b) in murine myeloid cells.  |  Schmidt, M., et al. 2004. Blood. 103: 4142-9. PMID: 14976051
4. Interferon-gamma induces regression of epithelial cell carcinoma: critical roles of IRF-1 and ICSBP transcription factors.  |  Egwuagu, CE., et al. 2006. Oncogene. 25: 3670-9. PMID: 16462767
5. PU.1 and ICSBP control constitutive and IFN-gamma-regulated Tlr9 gene expression in mouse macrophages.  |  Schroder, K., et al. 2007. J Leukoc Biol. 81: 1577-90. PMID: 17360957
6. Positive histone marks are associated with active transcription from a methylated ICSBP/IRF8 gene.  |  Tshuikina, M., et al. 2008. Gene. 410: 259-67. PMID: 18242011
7. ICSBP promoter methylation in myelodysplastic syndromes and acute myeloid leukaemia.  |  Otto, N., et al. 2011. Leukemia. 25: 1202-7. PMID: 21475251
8. The leukemia-associated fusion protein Tel-platelet-derived growth factor receptor β (Tel-PdgfRβ) inhibits transcriptional repression of PTPN13 gene by interferon consensus sequence binding protein (Icsbp).  |  Huang, W., et al. 2012. J Biol Chem. 287: 8110-25. PMID: 22262849
9. [Silencing of ICSBP/IRF8 expression in U226 cells and bone marrow mononuclear cells from patients with multiple myeloma].  |  Jia, G., et al. 2012. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 20: 1127-30. PMID: 23114132
10. The genomic structure of the murine ICSBP gene reveals the presence of the gamma interferon-responsive element, to which an ISGF3 alpha subunit (or similar) molecule binds.  |  Kanno, Y., et al. 1993. Mol Cell Biol. 13: 3951-63. PMID: 8321202

The preferred ICSBP antibody is ICSBP Antibody (E-9): sc-365042.